BIOLOGICAL VARIATION AMONG AFRICAN TRYPANOSOMES

描述(改编自申请人的摘要)：这是一辆来自Dr。 曼斯菲尔德，旨在确定非洲毒力的基础 锥虫。这项拟议的研究将延长最初由 一年R03奖，1997年制造，但通过免费延长到两年 分机。在这一点上，先前提案的目标基本上没有变化 然而，有一项报告称取得了更多进展。曼斯菲尔德博士假设 毒力可能由一种类似变阻器的机制控制，包括 差异表达的毒力基因(或抑制毒力的基因)。 有两个具体的目标：第一，识别差异转录的mRNAs 并在LouTat 1克隆的遗传相关成员中合成蛋白质 布氏锥虫的基因组结构及其特征 这些基因。其次，这些基因在毒力中的作用将得到证实。 通过基因互补、破坏对锥虫进行遗传操作 以及过度的表达。

DESCRIPTION (Adapted from the Applicant's Abstract): This is a new R21 from Dr. Mansfield that aims to determine the basis for virulence in African trypanosomes. The proposed research would extend studies originally funded in a one year RO3 award, made in 1997 but extended to two years via a no-cost extension. The aims of the previous proposal are essentially unchanged in this one, however, additional progress is reported. Dr. Mansfield hypothesizes that virulence may be controlled by a rheostat-like mechanism involving differentially expressed virulence genes (or genes that inhibit virulence). There are two specific aims: first to identify differentially transcribed mRNAs and synthesized proteins in genetically related members of the LouTat 1 clone of Trypanosoma brucei rhodesiense and to characterize the genomic organization of these genes. Second, the role of these genes in virulence would be confirmed by genetic manipulation of trypanosomes using gene complementation, disruption and over expression.