VAGINAL IMMUNITY ROLE OF ENDOGENOUS/EXOGENOUS AGENTS

内源性/外源性物质的阴道免疫作用

• 批准号: 2206562
• 负责人: WILLIAM H KUTTEH
• 金额: $ 2.43万
• 依托单位: UT SOUTHWESTERN MEDICAL CENTER
• 依托单位国家: 美国
• 财政年份: 1995
• 资助国家: 美国
• 起止时间: 1995-09-01 至 1996-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2206562
• 关键词: B lymphocyte; antibody formation; antifungal agents; biopsy; cellular immunity; contraceptives; cytokine; enzyme linked immunosorbent assay; estrogens; female; genital secretion; hormone regulation /control mechanism; human subject; humoral immunity; immunocytochemistry; menopause; menstrual cycle; mucosal immunity; pregnancy; spermicide; vagina; vagina disorder

The immune system of the female genital tract comprises the components of mucosal (local) and systemic immune compartments. The relative contributions from these two compartments of the immune system are influenced to a different degree by biological and endocrinological factors including the menstrual cycle, use of hormonal supplements, local contraceptives, and infection. Therefore, the long-term objectives of the proposed studies are to determine the impact of such factors on the origin and transport of antibodies in female genital tract secretions, and on the B and T lymphocytes associated with the genital tissues. To address these considerations, the following specific aims are proposed: 1) to evaluate the effect of the endogenous and exogenous hormonal status on the immune system of the genital tract by measuring the levels, isotypes, and molecular forms of mucosal antibodies in cervical mucus and vaginal secretions collected from women during their menstrual cycle, in pregnancy, and in the menopause; 2) to evaluate the effect of local estrogens, spermicides, and anti-fungal agents on genital tract immunity in women during their reproductive years before and after treatment with such agents; and 3) to evaluate the relative contributions of uterine cervical and vaginal B and T cells and their products to the immune system of the genital tract before and after specific mucosal immunization. The accumulated results will provide basic information concerning the immunology of the female reproductive tract. We will ascertain the specific effects of hormones and other exogenous agents on immunoglobulin, cytokine, and secretory component expression and production in the genital tract. Rational approaches for further studies on the enhancement of immunity in the genital tract should evolve. The effects of hormonal therapy, local spermicides, and other factors on the integrity, enhancement, or suppression of the immune system of the female genital tract will be addressed in these studies.

女性生殖道的免疫系统包括以下组成部分： 粘膜（局部）和全身免疫区室。 的相对 免疫系统的这两个部分的贡献是 在不同程度上受到生物学和内分泌学的影响， 因素包括月经周期，激素补充剂的使用，局部 避孕药和感染因此， 建议的研究是确定这些因素对原产地的影响， 女性生殖道分泌物中的抗体和运输，以及 与生殖器组织相关的B和T淋巴细胞。解决这些 考虑到这些因素，提出了以下具体目标：1）评估 内源性和外源性激素状态对免疫的影响 生殖道系统通过测量水平，同种型， 宫颈粘液和阴道粘膜抗体的分子形式 从女性月经周期中收集的分泌物， 妊娠期和绝经期; 2）评估局部 雌激素、杀精剂和抗真菌剂对生殖道免疫的影响 在育龄期妇女中， 这些药物;和3）评估子宫的相对贡献 宫颈和阴道B和T细胞及其产物对免疫系统的影响 在特异性粘膜免疫之前和之后的生殖道。 的 累积的结果将提供有关 女性生殖道的免疫学 我们将确定 激素和其他外源性试剂对免疫球蛋白的特异性作用， 细胞因子和分泌成分在生殖器中的表达和产生 道。进一步研究如何加强 生殖道的免疫力应该进化。荷尔蒙的影响 治疗、局部杀精等因素对精子的完整性， 增强或抑制女性生殖器的免疫系统 将在这些研究中进行讨论。