GANGLIONIC CONTROL OF MESENTERIC BLOOD VESSELS

肠系膜血管的神经节控制

• 批准号: 2216179
• 负责人: DAVID L KREULEN
• 金额: $ 12.44万
• 依托单位: WEST VIRGINIA UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1994
• 资助国家: 美国
• 起止时间: 1994-07-01 至 1996-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2216179
• 关键词: blood circulation; calcitonin gene related peptide; electrophysiology; evoked potentials; ganglions; gastrointestinal motility /pressure; guinea pigs; laboratory rat; mechanoreceptors; membrane activity; mesenteric artery; mesentery; mixed tissue /cell culture; muscarinic receptor; neural transmission; neuroanatomy; neuroeffector; neurons; neuropharmacology; neurotransmitters; vascular smooth muscle; vascular smooth muscle nervous control; vasoactive intestinal peptide; vasodilators; veins; voltage /patch clamp

The abdominal prevertebral ganglia consist of the celiac, superior mesenteric and inferior mesenteric ganglia and these ganglia provide the sympathetic innervation of the abdominal and pelvic blood vessels. The activity in these neurons controls the physiological state of the splanchnic vascular bed. The level of activity in these neurons is integrated from neural and hormonal inputs; these inputs include efferent sympathetic outflow, primary sensory neurons and special enteric and vascular sensory neurons. It is the aim of this research to determine the mechanisms whereby each of the inputs to ganglia control and modulate the mesenteric circulation. The findings from these studies will have implications not only for the mesenteric vascular bed but also for all vascular beds where neural control is operative. Using electrophysiological, anatomical and pharmacological techniques these studies will determine the following: 1) the characteristics of the sensory inhibitory junction potential (IJP) and associated vasodilatation evoked in mesenteric artery by intestinal distension, including which nerves mediate the response and neurotransmitter(s) involved; 2) the comparative pharmacology of mesenteric arteries and veins to elucidate the mechanisms underlying the greater responsiveness of mesenteric veins to nerve activation; 3) the physiological and pharmacological properties of cholinergic muscarinic e.p.s.p.s. evoked physiologically with intestinal distension; 4) biophysical properties of prevertebral ganglionic neurons determined with patch-voltage clamp in dissociated neurons; 5) ionic mechanism(s) of muscarinic depolarizations in dissociated ganglionic neurons determined with patch-voltage clamp and single electrode voltage clamp; 6) mechanism(s) of depolarizations produced in ganglionic neurons by candidate peptide neurotransmitters substance P, vasoactive intestinal peptide and calcitonin gene-related peptide; 7) characterize sympathetic nerve vascular smooth muscle interactions by evaluating neuromuscular transmission in neuron-smooth muscle co-cultures. The results of these studies will provide a fundamental understanding of the ways in which the sympathetic nervous system controls blood vessels and will provide rationale for the treatment of vascular and gastrointestinal disorders that have a neurogenic component including hypertension, ischemia, and inflammatory bowel disorders.

腹椎前神经节由腹上腹神经节组成。 肠系膜和肠系膜下神经节以及这些神经节为 腹部和盆腔血管的交感神经支配。这个 这些神经元的活动控制着大脑的生理状态 内脏血管床。这些神经元的活动水平是 从神经和荷尔蒙输入整合；这些输入包括传出 交感神经流出，初级感觉神经元和特殊的肠道和 血管感觉神经元。这项研究的目的是确定 神经节的每一个输入控制和调节 肠系膜循环。这些研究的结果将会有 不仅对肠系膜血管床，而且对所有 神经控制起作用的血管床。vbl.使用 电生理学、解剖学和药理学技术 研究将确定以下几个方面：1) 感觉抑制连接电位(IJP)与相关的血管扩张 肠扩张引起的肠系膜动脉反应，包括 神经调节反应和涉及的神经递质(S)；2) 肠系膜动、静脉的比较药理学研究 肠系膜静脉对血管的更强反应性的机制 神经激活；3)生理和药理特性。 胆碱能毒扁豆碱。肠道生理性诱发 4)椎前神经节神经元的生物物理特性 用膜片电压钳检测分离的神经元；5)离子 游离神经节细胞毒胆碱去极化机制(S) 膜片电压钳和单电极电压测定神经元 钳制；6)神经节神经元产生去极化的机制(S) 候选多肽神经递质P物质、血管活性肠肽 多肽和降钙素基因相关多肽；7)交感神经特性 评价神经肌肉功能的神经血管平滑肌相互作用 神经元-平滑肌共培养中的传递。这些研究的结果 研究将提供对以下方面的基本理解： 交感神经系统控制血管，并将提供 治疗血管和胃肠道疾病的理论基础 有神经源性成分，包括高血压、缺血和 炎症性肠病。