UTERINE METABOLISM AND BODY TEMPERATURE DURING DECIDUALIZED PSEUDOPREGNANCY

蜕膜假妊娠期间的子宫代谢和体温

• 批准号: 3789363
• 负责人: FITZGERALD SPENCER
• 金额: --
• 依托单位: SOUTHERN UNIV A&M COL BATON ROUGE
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3789363
• 关键词: albino rat; body temperature; decidua; dexamethasone; dosage; drug adverse effect; glycogen; medical education; minority institution research support; progesterone; progesterone receptors; protein metabolism; pseudopregnancy; telecommunications; uterus

The objective of this study will be to investigate whether dexamethasone (DEX), a synthetic glucocorticoid useful in chemical reproductive therapy in humans, can adversely affect two functions: namely, (1) cellular aspects of the uterine metabolism during decidualized pseudopregnancy (DCPG), thereby constituting a basis for the interruption of pseudopregnancy, and (2) the body temperature rhythm during this reproductive state. Biochemical endpoints (uterine weight, protein, glycogen, progesterone, cytosolic and nuclear progesterone receptors, and plasma progesterone) during deciduoma formation on Day 10 DCPG will be investigated to help clarify the possible mechanism of DEX action. The body temperature on Days 10-12 DCPG will be studied in order to evaluate the influence of DEX on this general function. The albino rat will be employed as the mammalian model. Rats, between 200-250 grams, will be maintained under controlled photoperiodic conditions of 12L:12D (lights on at 0500 hr., off at (700 hr.) and of ambient temperature (21 degrees +/- 2 degrees C). Those rates displaying at least two consistent 4 or 5 days estrous cycle (determined by vaginal lavages) will be used. After cervical stimulation with a vibrating plastic rod during proestrus and estrus, rats will undergo the induction of uterine deciduoma on the 4th day of continuous leucocytic infiltration in the vaginal lavages. This procedure will be surgically performed via laparotomy and by scratching the inner anti-mesometrial uterine membrane with a specially designed DeFeo needle. During the post-operative recovery period, rats, divided into three groups, will be subcutaneously injected with DEX (0.5,1.0 and 1.5 mg/rat) in daily doses for 5 days (Days 5-9 DCPG). Injections will occur at 0700 hr. (2 hours after the dark phase, and biochemical tests performed during the mid-light phase at 1100 hr. on Day 10 DCPG), and at 1500 hr. (2 hours before the dark phase, and biochemical tests conducted during the mid-dark phase at 2300 hr. on Day 10 DCPG). The body temperature will be continuously monitored and analyzed by means of a computerized biotelemetry system (Dataquest III) during Days 9-10 DCPG. Each group will consist of 10 animals. Results presented as means +/- standard errors, will be tested statistically, with a p value of a least 0.05 considered significant. Dose-response, Student's t, Analysis of Variance and the Cosinor regression methods will be employed for analyses of the data.

本研究的目的是调查地塞米松是否 (DEX)，一种用于化学生殖治疗的合成糖皮质激素 在人类中，可以对两种功能产生不利影响：即（1）细胞 蜕膜化假妊娠期间子宫代谢的各个方面 （DCPG），从而构成中断的基础 假怀孕，以及（2）假怀孕期间的体温节律 生殖状态。生化终点（子宫重量、蛋白质、 糖原、孕酮、胞质和核孕酮受体，以及 第 10 天蜕膜瘤形成过程中的血浆黄体酮 (DCPG) 将 研究有助于阐明 DEX 作用的可能机制。这 将研究 DCPG 第 10-12 天的体温以评估 DEX 对这个一般功能的影响。 白化老鼠将会 用作哺乳动物模型。 200-250克之间的老鼠 维持在12L:12D的受控光周期条件下（灯亮） 0500 小时，关闭（700 小时），环境温度（21 度+/- 2 摄氏度）。 这些价格显示至少两个一致的 4 或 5 天 将使用发情周期（通过阴道灌洗确定）。颈椎病后 在发情前期和发情期间用振动塑料棒刺激大鼠 将于产后第四天进行子宫蜕膜瘤诱导 阴道灌洗液中持续白细胞浸润。这个程序 将通过剖腹手术和刮擦内部进行手术 使用专门设计的 DeFeo 针来抗子宫内膜。 在术后恢复期间，将大鼠分为三组 各组皮下注射 DEX（0.5、1.0 和 1.5 mg/大鼠） 每日剂量，持续 5 天（DCPG 第 5-9 天）。 注射将于 0700 进行 小时。 （黑暗期后 2 小时，并在期间进行生化测试 1100 点的中光阶段。 DCPG 第 10 天）和 1500 小时。 （2小时 在黑暗阶段之前，以及在中黑暗期间进行的生化测试 2300 小时阶段。 DCPG 第 10 天）。体温将会是 通过计算机连续监测和分析 DCPG 第 9-10 天期间的生物遥测系统 (Dataquest III)。各组将 由10只动物组成。结果表示为平均值+/-标准误差， 将进行统计测试，考虑 p 值至少为 0.05 重要的。剂量反应、Student's t、方差分析和 将采用余弦回归方法来分析数据。