PHYSIOLOGICAL/BIOCHRONOMETRIC ANALYSIS OF 6-METHOXY-2-BENZOXAZOLINONE EXPOSURE

6-甲氧基-2-苯并恶唑啉酮暴露的生理学/生物计时分析

• 批准号: 6107040
• 负责人: FITZGERALD SPENCER
• 金额: $ 6.61万
• 依托单位: SOUTHERN UNIV A&M COL BATON ROUGE
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-01-01 至 1999-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6107040
• 关键词: albino rat; body temperature regulation; cervical /vaginal smear; circadian rhythms; estrus; metabolism; pregnancy; pseudopregnancy; radionuclides; sex cycle; statistics /biometry; telemetry; uterus

The broad objective of this study will be to investigate whether dexamethasone, a synthetic glucocorticoid useful in chemical reproductive therapy in humans, can indirectly affect ovarian function. Specifically, such influence on ovarian function will be tested via.the uterine metabolism and the body temperature rhythm during decidualized pseudopregnancy (DCPG) in albino rats. From a health related standpoint, dexamethasone, because of its suppression of androgens,is widely used to measure hypothalamic-hypophyseal-adrenal activity, and is also utilized in the chemical therapy of reproductive functions. Moreover, the study will be concerned with reproductive and body temperature functions in a mammalian species, the rat. Experimentally, rats between 200-250 grams, of the Sprague-Dawley strain will be employed. Rats displaying at least two consistent 4 or 5 days estrous cycle (determined by vaginal lavages) will be used. After cervical stimulation with a vibrating plastic rod during proestrus and estrus, rats will undergo the induction of uterine deciduoma (implantation simulation) on the fourth day of continuous leucocytic infiltration in the vaginal lavages. This procedure will be surgically performed via laparotomy and by scratching the inner anti-mesometrial uterine membrane with a specially designed needle. During the latter portion of a 5-8 days post-operative recovery period, rats will be divided into three groups of dexamethasone treatment. Rats will be subcutaneously injected with dexamethasone (0.5, 1.0, and 1.5 mg/rat) in daily doses for days post-implantationally (Days 6-10 DCPG) between 1000 to 1200 hours. Thereafter, the ovarian protein content and the uterine metabolism by way of biochemical endpoints (wet weight, protein and glycogen contents) will be examined by standard assay procedures on Day 11 of DCPG. The body temperature circadian rhythm will be monitored and analyzed by means of a computerized biotelemetry system (Dataquest III) during Days 11-16 of DCPG. Results, presented as means + standard errors, will be tested statistically, with a p value of at least 0.05 considered significant, by the Student's t-test, by the Analysis of Variance and by Cosinor regression analysis.

这项研究的主要目标是调查是否 地塞米松，一种用于化学生殖的合成糖皮质激素 对人类的治疗，可以间接影响卵巢功能。具体来说， 这种对卵巢功能的影响将通过子宫进行测试 蜕膜期的新陈代谢和体温节律 白化大鼠的假妊娠（DCPG）。从健康相关的角度来看， 地塞米松由于其抑制雄激素的作用而被广泛用于 测量下丘脑-垂体-肾上腺活动，也被利用 在生殖功能的化学治疗中。此外，该研究 将关注生殖和体温功能 哺乳动物物种，大鼠。实验上，200-250克之间的老鼠， 将使用Sprague-Dawley菌株。大鼠至少表现出 两个一致的 4 或 5 天动情周期（通过阴道灌洗确定） 将被使用。用振动塑料棒刺激宫颈后 在发情前期和发情期间，大鼠会进行子宫诱导 连续第四天的蜕膜瘤（植入模拟） 阴道灌洗液中有白细胞浸润。这个程序将是 通过剖腹手术和刮擦内部进行手术 用特殊设计的针抗子宫内膜。 在术后 5-8 天的恢复期后半段， 将大鼠分为三组，接受地塞米松治疗。老鼠 将皮下注射地塞米松（0.5、1.0和1.5 毫克/大鼠）植入后几天的每日剂量（DCPG第6-10天） 1000至1200小时之间。此后，卵巢蛋白质含量和 通过生化终点（湿重、 蛋白质和糖原含量）将通过标准测定进行检查 DCPG 第 11 天的程序。体温昼夜节律会 通过计算机生物遥测系统进行监测和分析 (Dataquest III) DCPG 第 11-16 天期间。结果以手段呈现 + 标准误差，将进行统计测试，p 值为 at 通过学生 t 检验，至少 0.05 被认为是显着的 方差分析和余弦回归分析。