UTERINE METABOLISM AND BODY TEMPERATURE RHYTHM DURING PSEUDOPREGNANCY

假妊娠期间的子宫代谢和体温节律

• 批准号: 3734147
• 负责人: FITZGERALD SPENCER
• 金额: --
• 依托单位: SOUTHERN UNIV A&M COL BATON ROUGE
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3734147
• 关键词: albino rat; body temperature regulation; cervical /vaginal smear; circadian rhythms; dexamethasone; embryo implantation; endometrium; estrus; female reproductive system surgery; glycogen; hormone regulation /control mechanism; hormone therapy; metabolism; monitoring device; protein metabolism; pseudopregnancy; reproductive system disorder chemotherapy; sex cycle; statistics /biometry; thermometry; uterus

The broad objective of this study will be to investigate whether dexamethasone, a synthetic glucocorticoid useful in chemical reproductive therapy in humans, can indirectly affect ovarian function. Specifically, such influence on ovarian function will be tested via.the uterine metabolism and the body temperature rhythm during decidualized pseudopregnancy (DCPG) in albino rats. From a health related standpoint, dexamethasone, because of its suppression of androgens,is widely used to measure hypothalamic-hypophyseal-adrenal activity, and is also utilized in the chemical therapy of reproductive functions. Moreover, the study will be concerned with reproductive and body temperature functions in a mammalian species, the rat. Experimentally, rats between 200-250 grams, of the Sprague-Dawley strain will be employed. Rats displaying at least two consistent 4 or 5 days estrous cycle (determined by vaginal lavages) will be used. After cervical stimulation with a vibrating plastic rod during proestrus and estrus, rats will undergo the induction of uterine deciduoma (implantation simulation) on the fourth day of continuous leucocytic infiltration in the vaginal lavages. This procedure will be surgically performed via laparotomy and by scratching the inner anti-mesometrial uterine membrane with a specially designed needle. During the latter portion of a 5-8 days post-operative recovery period, rats will be divided into three groups of dexamethasone treatment. Rats will be subcutaneously injected with dexamethasone (0.5, 1.0, and 1.5 mg/rat) in daily doses for days post-implantationally (Days 6-10 DCPG) between 1000 to 1200 hours. Thereafter, the ovarian protein content and the uterine metabolism by way of biochemical endpoints (wet weight, protein and glycogen contents) will be examined by standard assay procedures on Day 11 of DCPG. The body temperature circadian rhythm will be monitored and analyzed by means of a computerized biotelemetry system (Dataquest III) during Days 11-16 of DCPG. Results, presented as means + standard errors, will be tested statistically, with a p value of at least 0.05 considered significant, by the Student's t-test, by the Analysis of Variance and by Cosinor regression analysis.

本研究的主要目的是调查是否 地塞米松，一种用于化学生殖的合成糖皮质激素 在人类中的治疗，可以间接影响卵巢功能。具体地说， 这种对卵巢功能的影响将通过子宫 蜕膜化过程中的代谢和体温节律 在白化病大鼠中，从健康的角度来看， 地塞米松由于其对雄激素的抑制作用而被广泛用于 测量下丘脑-垂体-肾上腺活动， 生殖功能的化学治疗。此外，研究 将关注生殖和体温功能， 哺乳动物物种，老鼠。在实验中，200-250克的老鼠， 的Sprague-Dawley菌株。老鼠至少表现出 两个一致的4或5天发情周期（通过阴道灌洗确定） 将用于用振动塑料棒刺激颈部后 在发情前期和发情期，大鼠将经历子宫诱导， 蜕膜瘤（植入模拟）在连续第四天 阴道灌洗液中有白细胞浸润本程序将 通过剖腹手术并通过刮擦内部 用专门设计的针对子宫系膜进行穿刺。 在术后5-8天恢复期的后半部分， 将大鼠分为三组地塞米松治疗。大鼠 将皮下注射地塞米松（0.5、1.0和1.5 给药后（DCPG第6-10天）每日剂量（mg/大鼠） 在1000到1200小时之间。此后，卵巢蛋白质含量和 通过生物化学终点（湿重， 蛋白质和糖原含量）将通过标准测定法进行检查 DCPG第11天的程序。体温昼夜节律将 通过计算机化生物遥测系统进行监测和分析 （Dataquest III），DCPG第11-16天。结果，以平均值表示 + 标准误差，将进行统计学检验，p值为 通过Student t检验，通过 方差分析和余弦回归分析。