IN SITU RAMAN SPECTROSCOPIC ANALYSIS OF HUMAN ARTERIAL D

人体动脉 D 的原位拉曼光谱分析

• 批准号: 2227911
• 负责人: MICHAEL Stephen FELD
• 金额: $ 28.07万
• 依托单位: MASSACHUSETTS INSTITUTE OF TECHNOLOGY
• 依托单位国家: 美国
• 财政年份: 1993
• 资助国家: 美国
• 起止时间: 1993-12-01 至 1996-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2227911
• 关键词: Raman spectrometry; atherosclerosis; atherosclerotic plaque; biomedical equipment development; cardiovascular disorder diagnosis; charge coupled device camera; clinical biomedical equipment; computer assisted diagnosis; computer simulation; diagnosis design /evaluation; diagnosis quality /standard; diagnostic catheterization; fiber optics; fluorescence microscopy; histochemistry /cytochemistry; histopathology; human tissue; infrared spectrometry; laser spectrometry; light scattering; microspectrophotometry; photomicrography; prognosis; vascular smooth muscle

The long range goal of this proposal is to develop near infrared (NIR) Raman spectroscopy as a quantitative method for in situ detection of atherosclerosis and its progression in human peripheral arteries. With this technique, information on chemical composition and molecular identity can be obtained rapidly, accurately, and remotely. Therefore, it has the potential to provide quantitative measurement of histochemical and histopathological markers for both the presence and extent of atherosclerotic alterations. The specific goals of our proposal are to: l) Investigate and validate the use of NIR Raman spectroscopy for diagnosis and prognosis of atherosclerosis in muscular and elastic arteries. We propose several approaches for characterizing fully the spectroscopic variability of the MR Raman signals with disease-associated alterations of artery, and establishing the detection sensitivity to different biochemical moieties and metabolites. We have planed specific experiments to compare Raman and chemical assays, correlate the spectral features observed at different stages of atherosclerosis with the morphological and molecular changes that take place, and use this information to establish diagnostic decision schemes. 2) Develop vibrational spectroscopy techniques for clinical applications. Detailed experiments are outlined to improve NIR Raman systems for clinical applications and build new spectroscopic systems, including a portable system for use in an operating room and Raman microprobes. 3) Confirm the validity of spectral models and diagnostic algorithms in preclinical studies. An in vivo study of the tissue alterations caused by atherosclerosis is proposed with the objective of determining the transferability of the technologies developed in vitro. The technique generated by this investigation will be broadly applicable to the characterization of disease in various human arteries and, more broadly, other tissue systems, and will establish the capability for real- time in vivo determination of human tissue histochemistry and pathobiology.

该提案的长期目标是开发近红外（NIR） 拉曼光谱作为原位检测的定量方法 动脉粥样硬化及其在人类外周动脉中的进展。和 这项技术，有关化学成分和分子身份的信息 可以快速、准确、远程获取。因此，它具有 提供组织化学定量测量的潜力 组织病理学标记物的存在和程度 动脉粥样硬化改变。 我们提案的具体目标是： l) 调查并验证 近红外拉曼光谱在诊断和预后中的应用 肌性动脉和弹性动脉的动脉粥样硬化。我们提出几个 全面表征光谱变异性的方法 MR 拉曼信号显示与疾病相关的动脉变化，以及 建立对不同生化部分的检测灵敏度 和代谢物。我们计划了具体的实验来比较拉曼和 化学测定，将不同条件下观察到的光谱特征关联起来 动脉粥样硬化的各个阶段及其形态和分子变化 发生的情况，并使用此信息来制定诊断决策 计划。 2）开发临床振动光谱技术 应用程序。概述了改进近红外拉曼的详细实验 临床应用系统并构建新的光谱系统， 包括用于手术室的便携式系统和拉曼 微探针。 3）确认光谱模型和诊断的有效性 临床前研究中的算法。组织的体内研究 提出由动脉粥样硬化引起的改变，其目的是 确定体外开发的技术的可转移性。 这项研究产生的技术将广泛适用 各种人类动脉疾病的特征，以及更多 广泛地说，其他组织系统，并将建立真正的能力 体内时间测定人体组织化学和 病理学。