In Situ Raman Spectroscopic Analysis of Arterial Disease

描述(由申请人提供)：本提案的目标是开发一种 方法学，使用近红外(NIR)拉曼光谱作为定量 动脉粥样硬化及其进展的原位光谱分析方法 人体外周动脉。有了这项技术，动脉上的信息 可以快速、准确、准确地获得形态和化学成分 远程的。因此，它具有提供定量测量的潜力 组织形态和组织病理学标记物的存在和 动脉粥样硬化改变的程度。 该提案的具体目标是：1)执行基本的、量化的 拉曼光谱与动脉血管关系的研究 组织化学和形态观察。我们提出了几种方法来表征 全光谱变化的近红外拉曼信号与 与疾病相关的动脉改变。我们已经计划了具体的实验 将拉曼光谱与动脉形态进行比较，并利用这一信息 建立诊断决策方案。2)开发一类新的高度 手术室和手术室用灵敏近红外光纤光谱微型探头 临床环境。3)进行临床研究以评估新的探头设计 外周血管手术中的活体及存在的干扰 比如血。

DESCRIPTION (provided by applicant): The goal of this proposal is to develop a methodology, using near infrared (NIR) Raman spectroscopy, as a quantitative method, for in situ spectral analysis of atherosclerosis and its progression in human peripheral arteries. With this technique, information on the arterial morphology and chemical composition can be obtained rapidly, accurately and remotely. Therefore, it has the potential to provide quantitative measurement of histomorphological and histopathological markers for both the presence and extent of atherosclerotic alterations. The specific goals of the proposal are to: 1) perform basic, quantitative studies of the relationship between the Raman spectrum and the artery histochemistry and morphology. We propose several approaches for characterizing fully the spectroscopic variability of the NIR Raman signals with disease-associated alterations of artery. We have planned specific experiments to compare Raman spectra with artery morphology, and use this information to establish diagnostic decision schemes. 2) Develop a new class of highly sensitive NIR optical fiber spectral microprobes for use in operating room and clinical settings. 3) Perform clinical studies to evaluate new probe designs in vivo during peripheral vascular surgery and in the presence of interferences such as blood.