In Situ Raman Spectroscopic Analysis of Arterial Disease

描述（由申请人提供）：本提案的目标是开发一个 方法，使用近红外（NIR）拉曼光谱，作为定量 动脉粥样硬化及其进展的原位光谱分析方法 人体外周动脉通过这项技术，动脉上的信息 可以快速、准确地获得样品的形貌和化学成分， 远程控制因此，它具有提供定量测量的潜力 组织形态学和组织病理学标志物的存在和 动脉粥样硬化改变的程度。 该提案的具体目标是：1）执行基本的，定量的 拉曼光谱与动脉关系的研究 组织化学和形态学。我们提出了几种方法来表征 充分的光谱变化的近红外拉曼信号与 与疾病相关的动脉改变。我们已经计划了具体的实验 将拉曼光谱与动脉形态进行比较，并使用此信息 制定诊断决策方案。2)培养一个新的高度 用于手术室的灵敏近红外光纤光谱微探针， 临床环境。3)进行临床研究，以评估新的探头设计， 在外周血管手术期间和存在干扰的情况下 例如血液。

DESCRIPTION (provided by applicant): The goal of this proposal is to develop a methodology, using near infrared (NIR) Raman spectroscopy, as a quantitative method, for in situ spectral analysis of atherosclerosis and its progression in human peripheral arteries. With this technique, information on the arterial morphology and chemical composition can be obtained rapidly, accurately and remotely. Therefore, it has the potential to provide quantitative measurement of histomorphological and histopathological markers for both the presence and extent of atherosclerotic alterations. The specific goals of the proposal are to: 1) perform basic, quantitative studies of the relationship between the Raman spectrum and the artery histochemistry and morphology. We propose several approaches for characterizing fully the spectroscopic variability of the NIR Raman signals with disease-associated alterations of artery. We have planned specific experiments to compare Raman spectra with artery morphology, and use this information to establish diagnostic decision schemes. 2) Develop a new class of highly sensitive NIR optical fiber spectral microprobes for use in operating room and clinical settings. 3) Perform clinical studies to evaluate new probe designs in vivo during peripheral vascular surgery and in the presence of interferences such as blood.