Spectroscopic imaging and diagnosis of neoplasia

描述（由申请人提供）：这是一份向美国国立卫生研究院提交的关于建立生物工程研究伙伴关系的提案，旨在开发一种光谱成像方法，用于诊断侵袭前肿瘤（非典型增生）并监测其进展。该计划基于麻省理工学院光谱学实验室成功开发的光谱学临床仪器和相关诊断算法。即将开发的仪器将由两个部分组成，一个是基于光散射光谱i（LSS）的广域肿瘤成像系统，另一个是基于三模态光谱i（TMS）的光纤探针装置，用于研究由此揭示的可疑区域。该项目的目标是开发和完善新技术，并评估其在人类患者肿瘤进展的实时诊断、表征和治疗中的应用，口腔和子宫颈肿瘤病变的检测和监测将作为建立该技术潜力的模型系统。此外，还将开展基础研究，进一步提高该技术及其对侵袭前肿瘤的表征能力。将承担6个项目，每个项目由一名经验丰富的研究者领导。(1)开发、维护和完善用于临床研究的原型仪器和诊断算法。对(2)口腔和(3)宫颈疑似病变患者进行临床研究，评估和完善异常增生的诊断和监测技术，预测患者对化学预防和免疫治疗药物的反应。将开展两个旨在提高临床仪器诊断准确性的基础项目，一个(4)探索准多重散射光的使用，以提高LSS成像的灵敏度和深度分辨率，第二个(5)开发基于与疾病进展和消退相关的分子和细胞事件的新型光谱终点。(6)病理学支持活动将包括口腔和宫颈组织的分子标记分析，以及通过计算机辅助定量图像分析对同一活检组织的组织学切片进行分析。行政核心将协调项目的多学科活动，确保信息共享和有效沟通。这一合作伙伴关系由六个机构的专家调查员组成，其中包括受过物理和机械/电气工程培训的经验丰富的生物工程师、有癌症研究经验的病理学家，以及专门研究口腔和宫颈发育不良的医院临床医生。

DESCRIPTION (provided by applicant): This is a proposal to the National Institutes of Health to establish a Bioengineering Research Partnership to develop a spectroscopic imaging methodology for diagnosing pre-invasive neoplasia (dysplasia) and monitoring its progression. The proposed program is based on optical spectroscopic clinical instrumentation and associated diagnostic algorithms successfully developed at the MIT Spectroscopy Laboratory. The instrument to be developed will have two components, a system for wide-area imaging of neoplasia, based on light scattering spectroscopy i(LSS), and an optical fiber probe device for studying suspect regions thus revealed, based on tri-modal spectroscopy i(TMS). The goal of the program is to develop and perfect the new technology and assess its application to the diagnosis, characterization, and therapy of neoplastic progression in human patients in real time, The detection and monitoring of neoplastic lesions in the oral cavity and the cervix will be used as model systems for establishing the potential of the technology. In addition, basic studies to further improve the technology and its ability to characterize pre-invasive neoplasia will be conducted. Six projects will be undertaken, each led by an experienced investigator: (I) Prototype instruments and diagnostic algorithms for clinical studies will be developed, maintained and perfected. Clinical studies will be conducted on patients with suspected lesions in the (2) oral cavity and (3) uterine cervix to evaluate and perfect the technology for diagnosing and monitoring dysplasia and predicting the patient's response to chemopreventive and immunotherapeutic agents. Two basic projects aimed at enhancing the diagnostic accuracy of the clinical instrumentation will be undertaken, one (4) to explore the use of quasi-multiple scattered light to enhance the sensitivity and provide depth resolution to LSS imaging, and a second (5) to develop novel spectroscopic end-points based on well-characterized molecular and cellular events associated with the progression and regression of disease. (6) Pathology support activities will include analysis of oral and cervical tissues for molecular markers, and analysis of histologic sections of the same biopsy tissue by computer-assisted quantitative image analysis. An administrative core will coordinate the multidisciplinary activities of the program and insure information sharing and efficient communication. The partnership, composed of expert investigators at six institutions, will include experienced bioengineers with training in physics and mechanical/electrical engineering, pathologists experienced in cancer research, and hospital-based clinicians specializing in oral and cervical dysplasia.