MECHANISMS OF RED CELL AGING--HEMOLYTIC ANEMIA

红细胞衰老机制——溶血性贫血

• 批准号: 2225096
• 负责人: SERGIO S PIOMELLI
• 金额: $ 25.29万
• 依托单位: COLUMBIA UNIV NEW YORK MORNINGSIDE
• 依托单位国家: 美国
• 财政年份: 1992
• 资助国家: 美国
• 起止时间: 1992-02-01 至 1996-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2225096
• 关键词: 6 phosphofructokinase; adult human (21+); aging; blood tests; blood transfusion; cell age; cell type; chromatography; congenital blood disorder; congenital hemolytic anemia; congenital nonspherocytic hemolytic anemia; electrophoresis; enzyme mechanism; enzyme structure; erythrocyte membrane; erythrocytes; fluorescence spectrometry; glycolysis; hemolysis; hemolytic anemia; hexokinase; human tissue; isozymes; monoclonal antibody; posttranslational modifications; protoporphyria; pyruvate kinase; pyruvate kinase deficiency; thalassemia

This project aims at elucidating the pathophysiology of hemolytic anemias secondary to defects in red cell metabolism. We will investigate in detail the glycolytic process of the red cell "in vitro" utilizing newly developed techniques that allow metabolic flow to proceed unimpeded by metabolic block. This is a novel approach to the study of red cell metabolism, that shall permit analysis of glycolysis in detail, in conditions similar to those that exist in the red cell "in vivo". We will investigate in detail the biochemical changes that occur during the process of red cell aging "in vivo". We will examine, through analysis of glycolytic intermediates in age-fractionated red cells, the progressive development of metabolic alterations. We will study in great detail two key enzymes of glycolysis - hexokinase and pyruvate kinase. We will attempt to clarify their molecular structure and to assess the role played by isozymic shifts in the process of molecular aging of enzymes, versus the possibility that this may result from post-translational modifications.

本项目旨在阐明溶血性贫血的病理生理机制