AXON TARGET INTERACTIONS IN SENSORY MAP FORMATION

感官图形成中的轴突目标相互作用

• 批准号: 2270201
• 负责人: Reha S Erzurumlu
• 金额: $ 10.34万
• 依托单位: LSU HEALTH SCIENCES CENTER
• 依托单位国家: 美国
• 财政年份: 1994
• 资助国家: 美国
• 起止时间: 1994-05-01 至 1998-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2270201
• 关键词: afferent nerve; brain mapping; cell adhesion molecules; cell differentiation; central neural pathway /tract; developmental neurobiology; embryo /fetus tissue /cell culture; extracellular matrix; histochemistry /cytochemistry; laboratory rat; mixed tissue /cell culture; neocortex; neuronal guidance; neurotrophic factors; sensory receptors; thalamus; trigeminal nerve

This proposal addresses fundamental issues regarding the formation of sensory maps in the mammalian brain. Experiments are proposed to sort out the role of factors that are Intrinsic to growing axons vs. of those derived from their environment in a model in vitro system. The rodent trigeminal system is a highly specialized sensory system; it is characterized by a specific arrangement of axonal and neuronal elements which are homeomorphic to the distribution of sensory receptors (around whiskers) on the snout. The patterned distribution of sensory receptors, or the sensory map, is conveyed to the brain by primary sensory neurons which reside in the trigeminal ganglion. In this proposal, embryonic trigeminal ganglia will be co-cultured with a variety of peripheral target tissue and central nervous system explants. This recently developed technical approach will be used to experimentally test the role of target- derived influences, such as cell and substrate adhesion molecules, glial organization within target tissues, possible positional cues embedded in the target, and of growth factors that are manufactured in the targets to reveal many unknown facets of axon-target interactions which lead to the formation of sensory maps in the brain. Understanding cellular and molecular mechanisms which underlie the formation of neural pathways and the spatial order within their tracts and terminal zones is a major endeavor in developmental neurobiology. Such knowledge has powerful implications for our ability to repair damage in specific sensory pathways of the brain.

这项建议涉及到有关组建 哺乳动物大脑中的感觉地图提出实验来整理 轴突生长的内在因素与 在体外系统模型中从它们的环境中衍生。 啮齿动物三叉神经系统是一种高度专业化的感觉系统， 以轴突和神经元的特殊排列为特征 这是同胚的感觉受体的分布（周围 胡须）在鼻子上。感觉受体的模式分布， 或者说感觉地图，是由初级感觉神经元传递到大脑的 位于三叉神经节内在这份提案中， 三叉神经节将与多种外周靶共培养 组织和中枢神经系统外植体。最近开发的 技术方法将用于实验测试目标的作用， 衍生的影响，如细胞和底物粘附分子，神经胶质细胞， 组织内的目标组织，可能的位置线索嵌入在 目标，以及在目标中制造的生长因子， 揭示了轴突-靶相互作用的许多未知方面， 大脑中感觉地图的形成。 理解细胞和分子机制， 神经通路的形成和其束内的空间秩序， 终末区是发育神经生物学的一项重要奋进。等 知识对我们修复损伤的能力有着强大的影响， 大脑的特定感觉通路