U1 SNRNA GENE EXPRESSION AND TRANSCRIPTION

U1 SNRNA 基因表达和转录

• 批准号: 3755852
• 负责人: GRACIELA C CANDELAS
• 金额: --
• 依托单位: UNIVERSITY OF PUERTO RICO RIO PIEDRAS
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3755852
• 关键词: Arachnida; RNA biosynthesis; cell free system; computer assisted sequence analysis; gene expression; genetic regulation; genetic transcription; messenger RNA; molecular cloning; nucleic acid probes; nucleic acid sequence; polymerase chain reaction; protein biosynthesis; restriction mapping; secretory protein; small nuclear RNA

The long range aim of the project is to analyze the synthesis of a tissue- specific product using a suitable and fruitful model system. The immediate aim of this proposal is to investigate the timely tissue-specific accumulation of U1 small nuclear RNA (U1 snRNA) elicited by the stimulation into protein synthesis of fibroin producing glands (the model system). U1 snRNA plays a vital role in protein synthesis in the maturating messenger RNA. The reasons for these studies arises from evidence accrued thus far on the macromolecular syntheses which precede that of the secretory protein itself. Within these events, increased U1 snRNA accumulation occurs prior to the synthesis of mRNA. This accumulation, basis for the proposed project, is the result of an increase in the expression of the gene coding for U1 snRNA. Thus the study will be directed at the gene and its transcriptional activity. Isolation, cloning and characterization of the genes will lead to studies on transcription under cell-free conditions. Once the cell-free system is optimized, the testing of both cls and trans acting can be properly questioned, studies that will contribute towards the elucidation of the mechanism(s) underlying the event under analysis. The timeliness of this event is the fact that its production precedes that of its target, mRNA. U1 snRNA is a highly conserved molecule, thus information from our studies may be applicable to other forms of life, including humans. The relevance of the work lies in the conserved nature of U1 snRNA, the ubiquitousness of protein synthesis, and the role of U1 in the maturation of mRNA. Conservation is a high point of these molecules and its associated proteins (RNPs). Fungal snRNAs are bound specifically by Xenopus snRNP proteins. Epitopes recognized by several of the autoimmune antisera from lupus erythematosus patients have been conserved on snRNP proteins from humans to fungus.

该项目的长期目标是分析组织的合成 使用合适且富有成效的模型系统的特定产品。 即时的 该提案的目的是调查及时的组织特异性 刺激引起 U1 小核 RNA (U1 snRNA) 的积累 进入丝心蛋白产生腺体的蛋白质合成（模型系统）。 U1 snRNA 在成熟信使的蛋白质合成中起着至关重要的作用 核糖核酸。 这些研究的理由来自迄今为止积累的证据 关于先于分泌蛋白合成的大分子合成 本身。 在这些事件中，U1 snRNA 积累增加发生在 到 mRNA 的合成。 这种积累是提出建议的基础 项目，是编码基因表达增加的结果 对于 U1 snRNA。 因此，研究将针对该基因及其 转录活性。 分离、克隆和表征 基因将导致无细胞条件下转录的研究。 一旦无细胞系统被优化，cls 和 trans 的测试 行为可以受到适当的质疑，研究将有助于 阐明所分析事件的潜在机制。 这 该事件的及时性在于它的制作先于 它的目标，mRNA。 U1 snRNA 是一个高度保守的分子，因此 我们研究的信息可能适用于其他生命形式， 包括人类。 这项工作的相关性在于其保守性 U1 snRNA 的存在、蛋白质合成的普遍性以及 U1 在 mRNA 的成熟。 守恒是这些分子的一个亮点 及其相关蛋白（RNP）。 真菌 snRNA 特异性结合 爪蟾 snRNP 蛋白。 一些表位识别 红斑狼疮患者的自身免疫抗血清已被保存 从人类到真菌的 snRNP 蛋白。