TRANSFERRED RESISTANCE TO SCHISTOSOMES IN SNAILS

光肩星天牛先天抗性机制的研究 目前尚不清楚钉螺感染曼氏血吸虫，以及 具体地说，就是造血组织的作用，所谓 阿米巴产生器官(APO)在这种耐药中的作用尚不清楚。一个 最近的演示提出了解决这个问题的可能方法 同种异体APO移植后抗性的过继转移 基因抵抗型捐赠者。这个项目试图阐明 这种转移抗性的机制，即是否由于 产生血细胞(血细胞嵌合体)和/或分泌可溶性 “阻力系数”由植入物，并确定是否额外 蜗牛体内存在具有免疫活性的组织。载脂蛋白同种异体移植受者 将接受检查，看是否有丝分裂活动增加 血吸虫毛虫，循环血细胞数量增加， 供者血细胞DNA的存在和可溶性因子的存在 通常只在抗性血浆中发现，即酵母调理和 孢子囊凝集活性。额外的组织和器官 还将研究耐药蜗牛的转移能力。 血吸虫抗药性。最后，可能影响的几个变量 将检查转移的抵抗力：供体的年龄，供体的大小 植入物，以及植入物的激活状态。潜力 这项研究的意义在于，它可能有助于确定特定的 血吸虫与血吸虫先天抵抗力有关的器官、组织和细胞 感染。此外，如果血细胞嵌合体被证实， APO的造血功能将得到验证。

The mechanisms underlying innate resistance of Biomphalaria glabrata snails to infection with Schistosoma mansoni are not known, and specifically the role of the hematopoietic tissue, the so-called amoebocyte-producing organ (APO), in such resistance is unclear. A possible approach to this problem is suggested by the recent demonstration of adoptive transfer of resistance with allografts of the APO from genetically resistant donors. This project seeks to elucidate the mechanism for such transferred resistance, i.e., whether it is due to production of hemocytes (hemocytic chimerism) and/or secretion of soluble "resistance factors" by the implant, and to determine whether additional immunocompetent tissues are present in snails. APO allograft recipients will be examined for increased mitotic activity following exposure to schistosome miracidia, increased numbers of circulating hemocytes, presence of donor hemocyte DNA, and the presence of soluble factors normally found only in resistant plasma, i.e., yeast-opsonizing and sporocyst-agglutinating activity. Additional tissues and organs of resistant snails will also be studied for their ability to transfer schistosome resistance. Finally, several variables that may affect transferred resistance will be examined: age of the donor, size of the implant, and state of activation of the implant. The potential significance of this research is that it may help identify specific organs, tissues, and cells involved in innate resistance to schistosome infection. Furthermore, should hemocytic chimerism be demonstrated, the hematopoietic function of the APO will have been verified.