CHANGES IN CEREBRAL MICROCIRCULATION IN DEVELOPMENT

这项拟议的研究涉及脑血管的能力。 使它们的响应性、局部血流和结构适应 大脑皮层发育过程中大脑皮质的变化 对神经活动的反应，以及感觉去传入或 剥夺。我们使用成熟的模型系统，胡须-- 大鼠的桶状皮质。此协作结合了互补的 谢切诺夫地区脑血管实验室的人才与资源 中国科学院进化生理学与生物化学研究所 科学、圣彼得堡、俄罗斯共和国、苏联和华盛顿 密苏里州圣路易斯大学，美国。 这项提议将扩大和加强两个资助的研究项目。 美国国立卫生研究院：(A)ROlHL41075《脑血管生成模型》，和(B)R01 NS28781“大脑皮质活动时的脑血管成像”。会的 促进对当地脑血的合作和相关研究 Flow、P02、诱发活动、血管生成和血管重塑。 活体视频显微镜的方法、荧光示踪剂和 啮齿动物桶状皮质的组织学在圣彼得堡建立得很好。 路易。圣彼得堡的实验室提供专业知识 局部脑血流量的生物物理测量(ICBF)， 细胞外液体空间(ECS)和组织P02，使用同一桶 皮质模型。预期结果将包括以下各项的相关性 大鼠正常桶状皮质的生理和视频显微镜参数 大鼠在发育和发育过程中的脑血管重构 在选择性感觉神经去传入之后。

The proposed research addresses the ability of brain blood vessels to adapt their responsiveness, local blood flow and architecture to alterations in the cerebral cortex during cortical development, in response to neural activity, and after sensory deafferentation or deprivation. We use the well established model system, the whisker-- barrel cortex of the rat. This collaboration combines the complementary talents and resources of cerebrovascular laboratories in the Sechenov Institute of Evolutionary Physiology and Biochemistry, Academy of Sciences, St. Petersburg, Russian Republic, USSR, and in Washington University, St. Louis, Missouri, USA. This proposal will expand and enhance two research projects funded by NIH: (a) ROlHL41075 "Models for brain angiogenesis", and (b) R01 NS28781 "Imaging brain blood vessels during cortical activity". It will facilitate collaborative and correlative research on local cerebral blood flow, P02, evoked activity, angiogenesis, and vascular remodeling. Methods for videomicroscopy in vivo, fluorescent tracers, and histology of the barrel cortex of rodents are well established in St. Louis. The laboratory in St. Petersburg provides expertise in biophysical measurements of local cerebral blood flow (ICBF), extracellular fluid space (ECS), and tissue P02, using the same barrel cortex model. The expected results will include the correlation of physiological and videomicroscopic parameters in normal barrel cortex of rats and remodeling of cerebral blood vessels during development and after selective sensory deafferentation.