CHANGES IN CEREBRAL MICROCIRCULATION IN DEVELOPMENT

这项拟议中的研究涉及脑血管的能力 以适应他们的反应，局部血流和结构， 皮质发育过程中大脑皮质的变化， 对神经活动的反应，以及感觉传入阻滞后， 剥夺 我们使用已经建立的模型系统，胡须-- 大鼠的桶状皮质。 这种合作结合了互补的 谢切诺夫脑血管实验室的人才和资源 中国科学院进化生理学与生物化学研究所 科学、俄罗斯共和国彼得堡、苏联和华盛顿 美国密苏里州圣路易斯大学。 这项建议将扩大和加强两个研究项目的资助 NIH：（a）RO 1HL 41075“脑血管生成模型”，和（B）R 01 NS 28781“皮层活动期间脑血管成像”。 它将 促进局部脑血的合作和相关研究 血流、P02、诱发活性、血管生成和血管重塑。 用于体内视频显微镜检查的方法，荧光示踪剂，和 啮齿动物的桶皮质的组织学在St. Louis. 位于彼得堡的实验室提供以下专业知识： 局部脑血流量（ICBF）的生物物理测量， 细胞外液间隙（ECS）和组织P02，使用同一针筒 皮层模型 预期结果将包括以下方面的相关性： 生理和视频显微镜参数正常桶皮质 大鼠和脑血管重塑在发展和 在选择性感觉传入神经阻滞后

The proposed research addresses the ability of brain blood vessels to adapt their responsiveness, local blood flow and architecture to alterations in the cerebral cortex during cortical development, in response to neural activity, and after sensory deafferentation or deprivation. We use the well established model system, the whisker-- barrel cortex of the rat. This collaboration combines the complementary talents and resources of cerebrovascular laboratories in the Sechenov Institute of Evolutionary Physiology and Biochemistry, Academy of Sciences, St. Petersburg, Russian Republic, USSR, and in Washington University, St. Louis, Missouri, USA. This proposal will expand and enhance two research projects funded by NIH: (a) ROlHL41075 "Models for brain angiogenesis", and (b) R01 NS28781 "Imaging brain blood vessels during cortical activity". It will facilitate collaborative and correlative research on local cerebral blood flow, P02, evoked activity, angiogenesis, and vascular remodeling. Methods for videomicroscopy in vivo, fluorescent tracers, and histology of the barrel cortex of rodents are well established in St. Louis. The laboratory in St. Petersburg provides expertise in biophysical measurements of local cerebral blood flow (ICBF), extracellular fluid space (ECS), and tissue P02, using the same barrel cortex model. The expected results will include the correlation of physiological and videomicroscopic parameters in normal barrel cortex of rats and remodeling of cerebral blood vessels during development and after selective sensory deafferentation.