MOLECULAR BIOLOGY OF THE SYNAPSE

该研究计划的主要目标是对一种分子 定义，从而更深入地了解 Synapse。一个次要的目标，它利用了我们的试剂 在执行过程中的准备工作我们的主要目标是 了解神经元特异性基因是如何表达的 在空间和时间上有规律的。我们在此项目期间的目标 1.新的F1-20抗原的功能测定 突触特异的，发育调节的，神经元特异性蛋白 小鼠中枢神经系统。最近我们发现F1-20抗原是一种 神经元钙蛋白酶的底物。此外，我们最近还 确定F1-20抗原是一种磷蛋白。可能的 这些新结果对F1-20抗原功能的影响 也将进行探索。2.基因组DNA序列的初步评估 它们是实现准确的细胞类型特定的和暂时的 F1-20基因的调控。这将在转基因小鼠身上进行， 作为通向更长范围努力理解 神经细胞中基因表达的调控。3.对 F2-30抗原是一种新的神经元特异性蛋白，在 胚胎发育过程中中枢神经系统内的区域受限方式。我们会 完成我们对F2-30基因的描述，并检验假设 基于推导的氨基酸序列与III型之间的同源性 F2-30抗原在纤维连接蛋白结构域的建立中的作用 特定的突触连接。

The primary goal of the research program is to contribute to a molecular definition, and consequently deeper functional understanding of the synapse. A secondary goal which takes advantage of the reagents we are preparing in executing our primary goal is to contribute to an understanding of how the expression of neuronal specific genes are regulated spatially and temporally. Our objectives for this project period are: 1. Determination of the function of the F1-20 antigen, a novel synapse specific, developmentally regulated, neuronal specific protein in the murine CNS. Recently we have found that the F1-20 antigen is a substrate for neuronal calpain. Furthermore, we have also recently determined that the F1-20 antigen is a phosphoprotein. The possible implications of these new results for the function of the F1-20 antigen will also be explored. 2. Initial assessment of the genomic DNA sequences which are required to effect accurate cell-type specific and temporal regulation of the F1-20 gene. This will be carried out in transgenic mice, as the first step towards a longer range effort to understand the regulation of gene expression in neuronal cells. 3. Characterization of the F2-30 antigen, a novel neuronal specific protein expressed in a regionally restricted fashion within the CNS during embryogenesis. We will complete our characterization of the F2-30 cDNA, and test the hypothesis based n a homology between the deduced amino acid sequence with type III fibronectin domains that the F2-30 antigen play a role in the establishment of specific synaptic connections.