BIACORE 3000 SURFACE PLASMON RESONANCE INSTRUMENT

要求BIACORE表面等离子体共振（SPR）仪器支持圣安东尼奥德克萨斯大学健康科学中心（UTHSCSA）正在进行的大分子结构和动力学研究。UTHSCSA迫切需要SPR，这对我们用户群的数据收集需求至关重要。该仪器将支持需要最先进的SPR仪器来定义和表征各种研究问题中的大分子相互作用的研究。拟议的工具将大大加强对六个主要用户的研究。这一不同用户群体的共同特征包括：1)现场表面等离子体共振仪器的迫切需要，2)研究问题-致力于回答基本的生物学/生物物理学问题，以及3)资助的NIH研究项目对SPR有特定要求。该仪器将显著加强以下特定领域的研究：(1)包被囊泡动力学（Lafer），(2)染色质结构（Hansen），(3)黄素和血红素介导的酶系统（Masters），(4)细胞因子受体信号传导（Hinck），(5)金属酶结构和功能（Hart），以及(6)多亚单位酶系统（Henn）。拟议的文书得到该机构的大力支持。大学将提供资源来支付仪器维护和SPR实验室操作员的工资。SPR实验室将成为生物分子结构分析中心（CBSA）大分子动力学核心的重要组成部分。该核心的其他组成部分包括分析超离心，动态光散射，大颗粒凝胶电泳，微量热法和各种类型的紫外可见和荧光光谱。CBSA的其他核心实验室包括x射线晶体学实验室、核磁共振实验室、蛋白质实验室、质谱实验室和微生物发酵实验室。主要用户群中有分析超离心实验室（Hansen）、x射线晶体学实验室（Hart）和核磁共振实验室（Hinck）的主任，极大地促进了SPR实验室与中心其他互补技术之间的互动。

A BIACORE Surface Plasmon Resonance (SPR) instrument is requested to support the ongoing studies of macromolecular structure and dynamics at the University of Texas Health Science Center at San Antonio (UTHSCSA). There is an urgent need for SPR at the UTHSCSA that is essential to the data collection needs of our user base. This instrument will support research that requires state-of-the-art SPR instrumentation to define and characterize macromolecular interactions in a diverse array of research problems. The proposed instrument will greatly enhance the research of six major users. Common features of this diverse group of users include: 1) An immediate need for on-site surface plasmon resonance instrumentation, 2) Research problems- dedicated towards answering fundamental biological/biophysical questions, and 3) Funded NIH research programs which have specific requirements for SPR. Research in the following specific areas will be significantly enhanced by the proposed instrument: (1) Coated Vesicle Dynamics (Lafer), (2) Chromatin Structure (Hansen), (3) Flavin and Heme-Mediated Enzyme Systems (Masters), (4) Cytokine Receptor Signaling (Hinck), (5) Metaloenzyme Structure and Function (Hart), and (6) Multisubunit Enzyme Systems (Henn). The proposed instrument has the strong endorsement of the institution. The University will provide resources to cover instrument maintenance and the salary for the SPR Laboratory operator. The SPR Laboratory will be an essential component of the Macromolecular Dynamics Core of the Center For Biomolecular Structure Analysis (CBSA). Other components of this core include analytical ultracentrifugation, dynamic light scattering, large particle gel electrophoresis, microcalorimetry and various types of uv-vis and fluorescence spectroscopy. Other core laboratories in the CBSA include an X-Ray Crystallography Laboratory, Nuclear Magnetic Resonance Laboratory, Protein Laboratory, Mass Spectrometry Laboratory and Microbial Fermentation Laboratory. Among the major user group are the directors of the Analytical Ultracentrifugation Laboratory (Hansen), X-Ray Crystallography Laboratory (Hart), and Nuclear Magnetic Resonance Laboratory (Hinck), greatly facilitating interactions between the SPR Laboratory and the other complementary technologies of the Center.