PREPARATION OF MICROBICIDAL URINARY TRACT CATHETERS

目前，美国约有10%的医院患者接受 留置导尿管，这些导管导致超过40万 例与导尿管相关的尿路感染。 在 此外，这些导尿管刺激尿道表面， 插入，长期使用后的病变，以及增强的殖民化， 尿道表面的细菌。 抗生素的使用往往是一种 治疗无效;预防是最有希望的方法， 减少与器械相关的微生物感染。 的一种方法 防止细菌定植是通过将杀微生物剂附着到 植入前的器械。 Bio-Metric Systems，Inc.已经成功地衍生，纯化， 将抗微生物肽共价固定到聚苯乙烯上， 对保留的杀微生物活性进行了初步评估。 本研究中使用的肽，爪蟾抗菌肽，具有广谱活性 针对各种常见的传染性生物。 证明 magainin有效性的概念是通过制备 抗表皮葡萄球菌定植的改性表面 和大肠杆菌，如在体外测试的。 在这个项目中，我们 计划优化固定化化学和探索杀微生物剂 涂层尿路导管材料的体外活性。 第一阶段的具体目标是利用橡胶胶乳和硅橡胶 导尿管（作为两种最常用的导管材料）和： 1)将抗微生物肽涂布到每种导管类型上; 2）测量 抑制细菌定植;和3）开始开发程序， 结合润滑涂层以及杀微生物试剂 衬底表面。 II期研究将包括体外和体内研究 抗微生物活性、润滑性、组织损伤和沉积的测定 形成了一层硬壳

Currently, about 10% of the hospital patients in the United States receive indwelling urinary catheters, and these catheters lead to over 400,000 cases per year of catheter-associated urinary tract infections. In addition, these catheter irritate urethral surfaces, causing pain upon insertion, lesions after prolonged use, and enhanced colonization of the urethral surfaces by bacteria. Administration of antibiotics is often an ineffective treatment; prevention is the most promising approach to decreasing device-associated microbial infections. One approach to preventing bacterial colonization is by attaching microbicidal agents to the device prior to implantation. Bio-Metric Systems, Inc., has successfully derivatized, purified, and covalently immobilized an antimicrobial peptide to polystyrene and performed preliminary assessments of the retained microbicidal activities. The peptide used in this study, magainin, has broad spectrum activity against a variety of commonly occurring infectious organisms. Proof-of- concept for the effectiveness of magainin was demonstrated by preparing modified surfaces that resist colonization of Staphylococcus epidermidis and Escherichia coli, as tested in vitro. In this proposed project, we plan to optimize the immobilization chemistry and explore the microbicidal activity of coated urinary tract cathcter materials in vitro. Specific Phase I objectives are to utilize rubber latex and silicone rubber urinary catheters (as the two most commonly used catheter materials) and: 1) immobilize antimicrobial peptide onto each catheter type; 2) measure inhibition of bacteria colonization; and 3) begin developing procedures to incorporate lubricious coatings as well as microbicidal reagents on substrate surface. Phase II studies will include in vitro and in vivo assays for antimicrobial activity, lubricity, tissue damage and deposition of encrusting materials.