Pluses and minuses of lipid mass spectrometry

脂质质谱法的优点和缺点

• 批准号: DP190101486
• 负责人: Prof Stephen Blanksby
• 金额: $ 46.05万
• 依托单位: Queensland University of Technology
• 依托单位国家: 澳大利亚
• 项目类别: Discovery Projects
• 财政年份: 2019
• 资助国家: 澳大利亚
• 起止时间: 2019-01-01 至 2024-12-31
• 项目状态: 已结题
• 来源: https://dataportal.arc.gov.au/RGS/Web/Grants/DP190101486
• 关键词: Pluses; minuses; lipid; mass; spectrometry

This project aims to investigate the structural diversity of lipids. Lipids are among the most structurally diverse of all the biomolecules and thus deciphering their many functions requires bio-analytical technologies capable of uniquely identifying and quantifying individual molecules in a milieu of many thousands of analogues. Mass spectrometry is the pre-eminent technique for contemporary lipid analysis but is challenged by the preference of certain lipids to ionise with a polarity that affords sensitive detection but does not permit detailed structure elucidation. This project will develop advanced instrumentation capable of on-demand polarity switching of ionised lipids such that the detection and interrogation of molecular structure can take place in the optimal charge state.

本项目旨在研究脂质结构的多样性。脂质是所有生物分子中结构最多样化的，因此破译它们的许多功能需要生物分析技术，能够在成千上万的类似物中唯一地识别和量化单个分子。质谱分析是当代脂质分析的卓越技术，但受到某些脂质倾向于电离的挑战，这些脂质具有极性，可以提供敏感的检测，但不允许详细的结构阐明。该项目将开发先进的仪器，能够按需切换电离脂质的极性，以便在最佳电荷状态下进行分子结构的检测和询问。