HUMAN DECIDUA AND FETAL MEMBRANES AS A PARACRINE SYSTEM

人类蜕膜和胎膜作为旁分泌系统

• 批准号: 2389516
• 负责人: Gillian Doreen Bryant-Greenwood
• 金额: $ 23.34万
• 依托单位: UNIVERSITY OF HAWAII AT MANOA
• 依托单位国家: 美国
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-07-01 至 2001-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2389516
• 关键词: clinical research; collagen; collagenase; decidua; embryo /fetus membrane; enzyme activity; extracellular matrix; hormone receptor; human subject; northern blottings; paracrine; plasminogen activator; polymerase chain reaction; premature labor; protein metabolism; relaxin; stromelysin; tissue /cell culture; tissue inhibitor of metalloproteinases

The endocrinology of the reproductive system is based on systemic endocrine signalling and on the less readily intercepted signals between cells (paracrine) and within cells (autocrine and intracrine) which control local phenomena. The mechanism controlling parturition in women is a unique and local phenomenon. The most appropriate model for study, is the fetal/maternal interface, provided by the cells and extracellular matrix of the fetal amnion and chorion and the maternal decidua of the human fetal membranes. These are readily accessible, but only at a limited number of time points, for observation in vivo and experimentation in vitro. Hence, in normal tissues obtained at term, an autocrine/paracrine system has already been defined based on human relaxins and their control of specific metalloproteinases, an activator and inhibitor. The control of collagen breakdown and the finding of a novel elastic system in the amnion-chorion now explains fetal membrane accommodation and elasticity for the first time. Preterm premature rupture of the fetal membranes in the absence of infection resulting in preterm birth is a significant problem in perinatal medicine and can now be studied here by (1) seeking evidence in vivo for the excessive production of relaxins an-or expression of the relaxin receptor in women delivering preterm at 20-36 weeks gestation. (2) demonstrating a relationship between the increased relaxin/relaxin receptor expression and increased collagenolysis in vivo and in vitro, thereby linking relaxins and the preterm premature rupture of the fetal membranes. The combined results will show that the relaxins are involved in the preterm premature rupture of the fetal membranes and preterm birth: a significant problem in perinatal medicine in this country today.

生殖系统的内分泌学是基于系统的 内分泌信号和不易被截获的信号 细胞间（旁分泌）和细胞内（自分泌和内分泌） 控制着当地的现象。控制分娩的机制 是一种独特的地方性现象。 最合适的模型 用于研究的是胎儿/母体界面，由细胞提供， 胎儿羊膜和绒毛膜的细胞外基质和母体 人类胎膜的蜕膜。这些都很容易获得，但 仅在有限数量的时间点进行体内观察， 体外实验因此，在足月时获得的正常组织中， 自分泌/旁分泌系统已经基于人的 松弛素及其对特定金属蛋白酶的控制， 和抑制剂。胶原蛋白降解的控制和一种新的胶原蛋白的发现， 绒毛膜中的一种新的弹性系统现在解释了胎膜 适应性和弹性。预产期前过早 在没有感染的情况下胎膜破裂， 早产是围产期医学中一个重要问题， 现在可以通过以下方式进行研究：（1）在体内寻找过度 松弛素的产生和松弛素受体的表达 妊娠20-36周早产的妇女。(2)展示 松弛素/松弛素受体的增加 在体内和体外表达和增加胶原溶解，从而 将松弛素与胎儿早产 膜。综合结果将表明，松弛素是 涉及早产胎膜早破， 早产：这是围产期医学的一个重要问题， 国今日