The Aedes aegypti Genome Project

描述(由申请人提供)： 节肢动物传播的病毒性疾病在大多数热带国家再次成为主要的健康问题。例如，登革热的发病率在过去20年中大大增加，最近包括其出血性形式和相关的人类死亡。埃及伊蚊是主要的媒介。此外，埃及伊蚊是检验媒介与疟疾寄生虫(鸡疟原虫)和淋巴丝状线虫(马来丝虫)相互作用的极佳实验室模型。人类、黑腹果蝇、疟疾寄生虫媒介冈比亚按蚊、疟疾寄生虫恶性疟原虫和其他几种寄生虫基因组项目正在进行中。这项提议旨在启动埃及伊蚊基因组计划，期望对蚊媒、寄生虫和人类基因组的全面了解将促进和促进新的疾病控制战略。该项目的近期目标是：(1)对来自4个特定mRNA来源(从不同发育阶段和成人组织以及暴露于含有登革热病毒、疟疾寄生虫和丝虫寄生虫的受感染血粉中的雌性个体)产生的标准化文库中的40,000个cDNAs的两端进行测序；(2)对50,000个基因组DNA细菌人工染色体(BAC)克隆的两端进行测序；以及(3)使用荧光原位杂交(FISH)技术将1000个埃及按蚊BAC克隆物理定位到中期染色体上。这些目标将开发一系列基因组信息，包括EST序列、基因组序列和大型基因组克隆的物理图谱位置，这些信息将促进基因发现，并为最终的完整基因组测序项目提供关键工具。这项提议补充了一项即将完成的基因组计划，以检查主要的疟疾媒介冈比亚按蚊，因为这两个物种反映了蚊子物种之间基因组大小和组织的范围。长期目标是促进和促进资助机构之间的合作，以支持对埃及伊蚊整个基因组进行测序的努力。

DESCRIPTION (provided by applicant): Arthropod-borne viral diseases have re-emerged as major health problems in most tropical countries. For example, the incidence of dengue fever has increased greatly over the past 20 years, recently including its hemorrhagic form with associated human fatalities. The mosquito Aedes aegypti is the primary vector. In addition, A. aegypti is an excellent laboratory model for examining vector interaction with a malaria parasite (Plasmodium gallinaceum) and a lymphatic filarioid nematode (Brugia malayi). Whole genome sequence data is available for humans, Drosophila melanogaster, the malaria parasite vector Anopheles gambiae, the malaria parasite Plasmodium falciparum, and several other parasite genome projects are underway. This proposal seeks to initiate an A. aegypti genome project, with an expectation that complete knowledge of the mosquito vector, parasite and human genomes will promote and foster new disease control strategies. The immediate objectives of this project are to: (1) sequence both ends of 40,000 cDNAs derived from normalized libraries produced from 4 specific mRNA sources (from pooled mRNAs isolated from various developmental stages and adult tissues and from females exposed to infected blood meals containing dengue virus, a malaria parasite, and a filarial worm parasite), (2) to sequence both ends of 50,000 genomic DNA Bacterial Artificial Chromosome (BAC) clones, and (3) to use fluorescent in situ hybridization (FISH) technology to physically map 1000 A. aegypti BAC clones to metaphase chromosomes. These aims will develop a body of genome information including EST sequences, genomic sequences, and the physical map positions of large genomic clones that will enhance gene discovery, and also provide critical tools for an eventual complete genome sequencing project. The proposal complements a nearly completed genome project to examine the primary malaria vector, Anopheles gambiae, as the 2 species reflect the range of genome size and organization among mosquito species. The long range goal is to promote and foster collaboration between funding agencies to support an effort to sequence the entire A. aegypti genome.