Autogeny in Aedes aegypti

埃及伊蚊的自体繁殖

基本信息

批准号：
8020955
负责人：
DAVID W SEVERSON
金额：
$ 11.14万
依托单位：
UNIVERSITY OF NOTRE DAME
依托单位国家：
美国
项目类别：
财政年份：
2010
资助国家：
美国
起止时间：
2010-02-15 至 2013-01-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/8020955
关键词：
Address Aedes Automobile Driving Behavior Biochemical Pathway Biology Blood Candidate Disease Gene Cessation of life Chromosome Mapping Chromosomes Chromosomes, Human, Pair 3 Complex Culicidae Data Deltastab Dengue Dengue Virus Developed Countries Developing Countries Development Disease Disease Outbreaks Drug resistance Engineering Epidemic Event Evolution Experimental Designs Feeding behaviors Female Fingerprint Fostering Foundations Future Genes Genetic Genetic Markers Genome Genomics Goals Health Human Incidence Individual Insecticide Resistance Institutes Investigation Knowledge Libraries Microsatellite Repeats Modeling Molecular Molecular Genetics Mosquito Control Mosquito-borne infectious disease Outcome Ovarian Cycles Oviposition Parasites Phenotype Population Population Biology Positioning Attribute Production Protocols documentation Quantitative Genetics Quantitative Trait Loci Reporting Research Resolution Services Shotgun Sequence Assembly Single Nucleotide Polymorphism Single-Stranded Conformational Polymorphism Techniques Transgenes Travel Universities Vaccines Vitellogenesis Whole-Genome Shotgun Sequencing base disorder control disorder prevention egg genetic analysis genome sequencing genome wide association study novel pathogen physical mapping positional cloning prevent programs public health relevance research study tool trait transmission process vaccine development vector

项目摘要

DESCRIPTION (provided by applicant): Mosquito-borne diseases present an increasing global human health threat. These diseases reflect a significant resurgence in most developing countries, and also are emerging as clear threats for epidemic outbreaks in developed countries. Many factors contribute to the inability to date to prevent or control these diseases including poor progress in vaccine development, emergence of insecticide resistance in mosquito populations, drug resistance among parasite populations, lack of support in many developing countries for mosquito control programs, and increased global travel. This proposal seeks to initiate investigations on the autogeny trait observed in many mosquito species examined to date; autogenous females are able to produce an egg batch without the typical need for a blood meal as is typical with most female mosquitoes. Understanding the fundamental biology of mosquito populations, and particularly aspects of vitellogenesis, is critical to proposed efforts to utilize novel genetic approaches for controlling mosquito-borne diseases. At present, no knowledge exists on the molecular determinants of autogeny in any mosquito species. The focus of this proposal is the detailed genetic investigation of autogeny in Aedes aegypti as the model mosquito species for rapidly and efficiently uncovering the genetic basis for autogeny. Our long-term goals for these efforts are to eventually uncover genetic mechanisms likely common to autogeny determination in most if not all mosquito species. Aedes aegypti is the primary global vector for dengue virus. Dengue is a threat to >2.5 billion people, with an annual incidence of ~50 million cases and ~500,000 cases of DHF/DSS resulting in ~24,000 deaths per year. The objectives of this proposal are based on the general hypothesis that genes associated with the autogeny phenotype can be identified and isolated by positional cloning techniques. The proposed research will define a strong foundation toward the eventual detailed investigations of the genes determining autogeny and the knowledge obtained from the detailed understanding of the associated biochemical pathways will increase our basic understanding of vitellogenesis in mosquitoes which has implications for predicting vectorial capacity among mosquito populations. This could have several potential applications including transgene constructs that promote altered and disease limiting behaviors that significantly reduce vectorial capacity, development of targeted biopesticides, or identification of biologically relevant antagonists that disrupt behaviors critical to pathogen transmission or survival. The research plan builds upon and enhances the information obtained from the Ae. aegypti genome project to develop the necessary tools toward the eventual complete elucidation of these gene relationships and their phenotypic outcome. The specific aims of the project are to: 1) perform fine-scale genetic mapping to identify regions containing genes that determine the autogeny phenotype in Aedes aegypti and 2) conduct high-resolution, fine-scale physical mapping of an Aedes aegypti BAC library. PUBLIC HEALTH RELEVANCE: Aedes aegypti is the primary vector for dengue virus. No vaccines are available and disease prevention is largely dependent on avoiding contact with infected mosquitoes. The proposed research will provide valuable new information on the biology of this mosquito relating to the ability of some females to produce eggs without obtaining a blood meal.

描述（由申请人提供）：蚊子传播的疾病呈现出越来越多的全球人类健康威胁。这些疾病反映了大多数发展中国家的重大复兴，并且也正成为发达国家流行病爆发的明显威胁。许多因素导致无法预防或控制这些疾病，包括疫苗发育的进展不佳，蚊子种群中杀虫剂耐药性的出现，寄生虫种群中的耐药性，许多发展中国家对蚊子控制计划缺乏支持以及增加全球旅行。该提案旨在开始对迄今为止在许多蚊子物种中观察到的自动性状进行调查；大多数雌性蚊子典型的妇女无需典型的血液餐就可以产生鸡蛋批次。了解蚊子种群的基本生物学，尤其是叶片发生的方面，对于提出了利用新型遗传方法来控制蚊子传播疾病的努力至关重要。目前，尚无关于任何蚊子物种中自动基因的分子决定因素的知识。该提案的重点是伊德斯埃及埃及自动基因的详细遗传研究是迅速有效地发现自动源的遗传基础的模型蚊子物种。我们对这些努力的长期目标是最终发现大多数蚊子物种（即使不是全部）可能是自动确定常见的遗传机制。埃及埃及是登革热病毒的主要全球媒介。登革热是对> 25亿人的威胁，每年发生约5000万例DHF/DSS病例，每年约24,000例死亡。该提案的目标是基于以下一般假设：可以通过位置克隆技术鉴定和隔离与自动表型相关的基因。拟议的研究将为确定自动基因的基因以及从对相关生化途径的详细理解中获得的知识的最终详细研究定义一个强大的基础，这将增加我们对蚊子中对植物生成的基本理解，这对预测蚊子种群中的媒介物有影响。这可能具有多种潜在的应用，包括促进改变和疾病的行为的转基因构建体，这些构造可显着降低媒介物能力，靶向生物农药的发展或鉴定生物学相关的拮抗剂，这些拮抗剂破坏了对病原体传播或生存至关重要的行为。研究计划基于并增强了从AE获得的信息。 Aegypti基因组项目旨在开发必要的工具，以最终完全阐明这些基因关系及其表型结果。该项目的具体目的是：1）执行精细的遗传学映射，以识别含有基因的区域，这些区域确定了埃及埃及中的自源表型和2）进行高分辨率的高分辨率，精细的物理映射。公共卫生相关性：埃及埃及是登革热病毒的主要向量。没有可用的疫苗，预防疾病在很大程度上取决于避免接触被感染的蚊子。拟议的研究将提供有关该蚊子生物学的有价值的新信息，该信息与某些女性产生鸡蛋的能力而无需获得血液粉。