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Diapause in Culex Mosquitoes

库蚊滞育

基本信息

批准号：
8466274
负责人：
DAVID W SEVERSON
金额：
$ 56.12万
依托单位：
UNIVERSITY OF NOTRE DAME
依托单位国家：
美国
项目类别：
财政年份：
2009
资助国家：
美国
起止时间：
2009-06-01 至 2015-05-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/8466274
关键词：
Address Adult Arboviruses Area Behavior Biochemical Pathway Biological Assay Biology Candidate Disease Gene Climate Complex Culex (Genus)Culex pipiens Culicidae Data Analyses Developed Countries Developing Countries Development Diapause Disease Disease Outbreaks Drug resistance Epidemic Experimental Designs Female Filaria bancrofti Filarial Elephantiases Foundations Gene Expression Profile Gene Expression Regulation Genes Genetic Genetic Crosses Genetic Engineering Genetic Markers Genetic Transcription Genome Genome Mappings Genomics Geographic Distribution Goals Health Human Individual Insecticide Resistance Knowledge Laboratories Lymphatic Mediating Microarray Analysis Microsatellite Repeats Molecular Molecular Genetics Molecular Profiling Mosquito Control Mosquito-borne infectious disease Nematoda North America Parasites Pattern Phenotype Population Population Replacements Procedures Production Protocols documentation Quantitative Trait Loci RNA Interference Recombinants Relative (related person)Research Restriction fragment length polymorphism Single Nucleotide Polymorphism Single-Stranded Conformational Polymorphism Surveys Techniques Time Transgenes Travel Vaccines Validation Virus West Nile virus base disease transmission disorder control disorder prevention genome sequencing genome-wide novel overexpression pathogen positional cloning programs research study trait transmission process vaccine development vector

项目摘要

Project Summary Mosquito-borne diseases represent major health problems in most developing countries and have emerged as significant threats for epidemic outbreaks in developed countries. Exacerbating these problems are the emergence of insecticide resistance in mosquito populations, drug resistance among parasite populations, delayed progress in vaccine development, lack of support for mosquito control programs, and increased global travel. Mosquitoes in the Culex pipiens complex are primary vectors for the lymphatic filarioid nematode parasite, Wuchereria bancrofti and several arboviruses. This includes the recently introduced West Nile virus in North America. The long- term objectives of the proposed research are to identify, isolate and characterize genetic factors associated with diapause, a trait that strongly impacts the geographic distribution and survival of species within the Culex pipiens complex. At present, no knowledge exists on the molecular determinants of diapause in Culex. The objectives of this proposal are based on the general hypothesis that genes associated with the diapause phenotype can be identified and isolated by positional cloning techniques. Identification of genes determining diapause and the knowledge from the detailed understanding of the associated biochemical pathways could have several potential applications including: transgene constructs that promote altered and disease limiting behaviors following population replacement, development of targeted biopesticides, or identification of biologically relevant antagonists that disrupt behaviors critical to pathogen transmission or survival. The specific aims of the project are to: 1) fine-scale map genome regions identified as containing genes that determine the diapause phenotype, 2) identify candidate genes determining diapause, and 3) conduct functional analysis of candidate genes determining diapause. Information from genetic crosses will be leveraged against the Cx. quinquefasciatus genome project to identify candidate genes within discrete genome regions. Candidate genes will be evaluated using microarrays, followed by quantitative real time PCR assays to confirm and expand microarray results for promising genes. Positive candidate genes will be assayed for biological relevance using RNA interference mediated gene knockdowns or gene overexpression using recombinant Sindbis transducing viruses. Results from laboratory experiments will then be evaluated among natural populations representing Cx. pipiens s.s. and Cx. quinquefasciatus.

项目总结