CORE--MOLECULAR ANALYSIS

核心--分子分析

• 批准号: 6240758
• 负责人: FRANCES SMITH
• 金额: $ 13.34万
• 依托单位: EUNICE KENNEDY SHRIVER CTR MTL RETARDATN
• 依托单位国家: 美国
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-08-01 至 1998-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6240758
• 关键词: biomedical facility; mass spectrometry; mental retardation; molecular biology; nucleic acid sequence; polymerase chain reaction; protein purification

Molecular approaches allow investigators to delineate the mechanisms that implement the normal developmental program of each cell, and pathologic processes that cause development to go awry. This Core has been configured to facilitate implementation of these approaches. It provides investigators with the equipment, materials, and expertise needed to efficiently identify and characterize molecules of interest, and to manipulate the expression of these molecules to ask biologically relevant questions. The core has two major components; Biology Facility, which incorporates a newly established Protein Isolation Unit, and the Mass Spectrometry Facility. Each component provides a key service for analyzing complex molecular mixtures. The Molecular Biology Facility will provide capabilities for and instruct users in polymerase chain reaction (PCR) amplification of nucleic acid sequences, subcloning and amplification of plasmids, analysis of nucleic acids by agarose and acrylamide electrophoresis, Northern and Southern blotting, and DNA sequencing. The facility will serve as a common source for vectors and bacterial strains, and provide pretested competent cells for routine subcloning procedures. Molecular biological reagents such as restriction enzymes, DNA and RNA modifying enzymes, autoradiographic film, polaroid film, and transfection reagents will be obtained at bulk rates. The Protein Isolation Unit offers (a) the capability for employing sophisticated methodology for protein isolation and characterization, and (b) instruction in these techniques. Protein isolation and characterization is often closely related to the nucleic acid work carried out in the Molecular Biology Facility; it is frequently a necessary first step in the cloning of genes that encode proteins of biological importance. The Mass Spectrometry Facility provides investigators with a collaborative research resource that has the expertise and equipment to provide a wide range of mass spectrometry services, including structure determination, measurement of sample components, metabolite profiling and stable isotope tracer studies. Facility personnel have expertise in the analytical chemistry and biochemistry of glycoconjugates, lipids, peptides, bile acids, and amino acids.

分子方法使研究人员能够描绘出以下机制： 执行每个细胞的正常发育程序，以及病理 导致发展出错的流程。 该核心已配置 促进这些方法的实施。 它提供 调查人员拥有所需的设备、材料和专业知识 有效地识别和表征感兴趣的分子，并 操纵这些分子的表达来询问生物学相关性 问题。 核心有两个主要组成部分； 生物设施，其中 包含一个新建立的蛋白质分离装置，以及质量 光谱测量设施。 每个组件都提供关键的分析服务 复杂的分子混合物。 分子生物学设施将提供 聚合酶链式反应 (PCR) 的功能并指导用户 核酸序列的扩增、亚克隆和扩增 质粒，琼脂糖和丙烯酰胺核酸分析 电泳、Northern 和 Southern 印迹以及 DNA 测序。 这 该设施将作为载体和细菌菌株的共同来源， 并为常规亚克隆程序提供预先测试的感受态细胞。 分子生物试剂，如限制性内切酶、DNA 和 RNA 修饰酶、放射自显影胶片、宝丽来胶片和转染 试剂将以散装价格获得。 蛋白质分离装置提供 (a) 采用复杂的蛋白质方法的能力 分离和表征，以及（b）这些技术的指导。 蛋白质的分离和表征通常与 在分子生物学设施中进行的核酸工作；这是 通常是克隆编码基因的必要的第一步 具有生物学重要性的蛋白质。 质谱设施提供 具有专业知识的协作研究资源的研究人员 和设备来提供广泛的质谱服务， 包括结构测定、样品成分测量、 代谢物分析和稳定同位素示踪剂研究。 设施人员 拥有分析化学和生物化学方面的专业知识 糖复合物、脂质、肽、胆汁酸和氨基酸。