SLOW WAVE SLEEP--CONTROL BY THERMOREGULATORY NEURONS

描述（改编自申请人的摘要）： 睡眠是一种重要的睡眠方式，睡眠的功能是最重要的。 拟议的研究将评估有关行为的预测， 睡眠活跃的无线传感器网络记录在自由移动的大鼠。 一是因为 昼夜节律对睡眠的影响这些神经元被预测会表现出变化 与睡眠中的昼夜节律相关的兴奋性和活动 倾向 第二，由于睡眠具有强大的自我平衡调节功能， 预计这些神经元表现出增加的活性和兴奋性， 睡眠不足后。 我们还将绘制 这些神经元。 含有抑制性神经递质的神经元， γ-氨基丁酸（GABA）分布在POAH和邻近的 包含WSNs的站点。 有证据表明，增加GABA的释放， NREM睡眠。 POAH无线传感器网络具有抑制输出。 我们将评估 假设假定催眠睡眠活跃WSN是GABA能的，使用 麻醉动物的细胞内标记和GABA的免疫染色。 如果这些假设得到证实，我们就可以构建一个详细的NREM模型 睡眠功能的控制和指示。 睡眠障碍 诸如抑郁症和嗜睡症之类的异常， 机械术语

DESCRIPTION (Adapted from applicant's abstract): The brain mechanisms that generate sleep and the functions served by sleep remain among the great Proposed studies will assess predictions concerning the behavior of sleep-active WSNs recorded in freely-moving rats. First, because of the circadian influences on sleep these neurons are predicted to exhibit changes in excitability and activity correlated with circadian rhythms in sleep propensity. Second, because of the potent homeostatic regulation of sleep, these neurons are predicted to show increased activity and excitability after sleep deprivation. We will also map the anatomical distribution of these neurons. Neurons containing the inhibitory neurotransmitter, gama-aminobutyric acid (GABA) are distributed within the POAH and adjacent sites containing WSNs. There is evidence for increased GABA release during NREM sleep. POAH WSNs have inhibitory outputs. We will assess the hypothesis that putative hypnogenic sleep-active WSNs are GABAergic, using intracellular labeling in anesthetized animals and immunostaining for GABA. If these hypotheses are confirmed, we can construct a detailed model of NREM control and indications of sleep function. Disorders involving sleep abnormalities such as depression and narcolepsy may then be understood in mechanistic terms.