SLOW WAVE SLEEP--CONTROL BY THERMOREGULATORY NEURONS

描述(摘自申请者的摘要)：大脑机制 产生睡眠，而睡眠所起的作用仍然是最大的 拟议的研究将评估关于 在自由活动的大鼠中记录到睡眠活跃的WSNs。首先，因为 昼夜节律对睡眠的影响这些神经元预计会出现变化 兴奋性和活动度与睡眠的昼夜节律相关 倾向性。其次，由于睡眠对体内平衡的强大调节作用， 据预测，这些神经元表现出更强的活性和兴奋性。 在睡眠不足之后。我们还将绘制其解剖分布图 这些神经元。含有抑制性神经递质的神经元， 伽马-氨基丁酸(GABA)分布在POAH内及其附近 包含无线传感器网络的站点。有证据表明，在 NREM睡眠。POAH无线传感器网络具有抑制输出。我们将评估 假设催眠睡眠活跃的无线传感器网络是伽马酸能的，使用 麻醉动物细胞内标记和GABA免疫染色。 如果这些假设得到证实，我们就可以构建一个详细的NREM模型 睡眠功能的控制和指示。与睡眠有关的障碍 抑郁症和发作性睡病等异常可能会被理解为 机械术语。

DESCRIPTION (Adapted from applicant's abstract): The brain mechanisms that generate sleep and the functions served by sleep remain among the great Proposed studies will assess predictions concerning the behavior of sleep-active WSNs recorded in freely-moving rats. First, because of the circadian influences on sleep these neurons are predicted to exhibit changes in excitability and activity correlated with circadian rhythms in sleep propensity. Second, because of the potent homeostatic regulation of sleep, these neurons are predicted to show increased activity and excitability after sleep deprivation. We will also map the anatomical distribution of these neurons. Neurons containing the inhibitory neurotransmitter, gama-aminobutyric acid (GABA) are distributed within the POAH and adjacent sites containing WSNs. There is evidence for increased GABA release during NREM sleep. POAH WSNs have inhibitory outputs. We will assess the hypothesis that putative hypnogenic sleep-active WSNs are GABAergic, using intracellular labeling in anesthetized animals and immunostaining for GABA. If these hypotheses are confirmed, we can construct a detailed model of NREM control and indications of sleep function. Disorders involving sleep abnormalities such as depression and narcolepsy may then be understood in mechanistic terms.