权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

NEUROBIOLOGICAL STUDY OF VOCALIZATION

发声的神经生物学研究

基本信息

批准号：
2033822
负责人：
MEI-FANG CHENG
金额：
$ 14.29万
依托单位：
RUTGERS THE STATE UNIV OF NJ NEWARK
依托单位国家：
美国
项目类别：
财政年份：
1990
资助国家：
美国
起止时间：
1990-08-01 至 1999-05-31
项目状态：
已结题

项目摘要

The long term objective of this project is to delineate mechanisms by which behavior influence brain structure and function. Our strategy is to focus on a well characterized behavior with known adaptive functions - the endocrine-stimulating vocal behavior. This proposal is an extension of a previous finding that neurosecretory hypothalamus receives axonal projections from the vocal control midbrain and the marginal auditory thalamus. We now propose to further these findings by verifying acoustic properties, characterizing neurochemical properties of these pathways, and exploring the functional role of these projections. We will be using antidromically evoked extra-cellular recordings, anterograde tracers, retrograde tracers and immunohistochemical double- labeling methods to test the hypothesis that the afferent and efferent projections of the accessory auditory thalamus that we have discovered are in fact acoustic responsive, met-enkephalin positive pathways. We further postulate that estrogen receptors co-localize with (1) the met- enkephalin positive cells in the midbrain that give rise to terminal projection into the accessory auditory thalamus and (2) the met- enkephalin positive cell groups in the marginal thalamus. Finally, we will use opioid receptor agonist and antagonists, ovariectomy and hormone replacement study to explore the role of enkephalin and estrogen in the vocal signals induced endocrine changes. The proposed studies will provide the neural mechanisms whereby an individual's own vocal behavior and environmental sounds can influence the endocrine state of the individual. It is hoped that this line of work will serve as a model for future research on the subject of acoustic environment and health issues.

该项目的长期目标是通过以下方式界定机制：哪些行为影响大脑结构和功能。我们的策略是专注于具有已知适应性功能的明确特征行为 - 刺激内分泌的声音行为。该提议是先前发现的延伸，即神经分泌下丘脑接收来自声音控制中脑的轴突投射和边缘听觉丘脑。我们现在建议进一步推进这些通过验证声学特性、表征神经化学的发现这些途径的特性，并探索这些途径的功能作用预测。我们将使用逆向诱发的细胞外记录，顺行示踪剂、逆行示踪剂和免疫组化双标记方法来检验传入和传出的假设我们发现的副听觉丘脑的投射事实上，它们是声学反应、甲硫氨酸脑啡肽正向通路。我们进一步假设雌激素受体与（1）met-共定位中脑中的脑啡肽阳性细胞产生终末期投射到副听觉丘脑和（2）met- 边缘丘脑中脑啡肽阳性细胞群。最后，我们将使用阿片受体激动剂和拮抗剂、卵巢切除术和激素替代研究探讨脑啡肽和雌激素在声音信号引起内分泌变化。拟议的研究将提供神经机制，从而个人自身的声音行为和环境声音会影响个人的内分泌状态。希望这一行的工作将作为未来声学研究的模型环境和健康问题。