Brain Damage and Recovery of Function in the Adult System

成人系统的脑损伤和功能恢复

• 批准号: 7485615
• 负责人: MEI-FANG CHENG
• 金额: $ 16.76万
• 依托单位: RUTGERS THE STATE UNIV OF NJ NEWARK
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-09-13 至 2010-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7485615
• 关键词: Address; Adult; Ara-C; Area; Behavior; Behavioral; Bilateral; Brain; Brain Injuries; Bromodeoxyuridine; Castration; Cell Nucleus; Cells; Characteristics; Columbidae; Complex; Courtship; Cytosine; Disruption; Endocrine; Exhibits; Female; Fire - disasters; Generations; Goals; Housing; Hypothalamic structure; Immunohistochemistry; Label; Lesion; Measures; Mediating; Midbrain structure; Mitotic; Modeling; Monitor; Motor; Motor Pathways; Nature; Nesting Behavior; Neurons; Neuropeptides; Neurotransmitters; Newborn Infant; Obstruction; Output; Pattern; Performance; Physiological; Property; Radioimmunoassay; Recovery; Recovery of Function; Recruitment Activity; Role; Site; Stimulus; Stroke; Sum; System; Tactile; Testing; Testosterone; Therapeutic; Tracer; Visual; Work; behavior observation; male; neurochemistry; neurogenesis; response; social

DESCRIPTION (provided by applicant): Our long-term goal is to determine how to help adult victims of brain injury recover the function of brain areas damaged by stroke or other afflictions. To that end, the goal of this study is to determine the mechanisms that underlie the recovery of function after damage to the adult brain. The present proposal builds upon two conceptually complementary observations. We have observed that the recovery of complex courtship behavior (the nest coo) after bilateral hypothalamic lesion in the adult male ring dove is facilitated when a male ring dove is housed with a female. Secondly, after electrolytic lesions of the adult hypothalamus, newborn neurons were detected at the lesion site concurrent with recovery at the lesion site of units exhibiting normal firing responses to female nest coo stimulation and behavioral recovery 2-3 months after lesion. These observations suggest an alternative explanation to the widely accepted principle of recovery of function. Recovery might be mediated not only by existing, undamaged neurons, but also by lesion-induced new neurons recruited into the network of reorganization. We test the hypothesis that lesion-induced new neurons are involved in the recovery of physiological activity and nest coo behavior by blocking neurogenesis and observing the effects on recovery (Aim I) and the determining whether the new neurons are biologically functional, namely, whether they are integrated into the network of nest coo behavior. This will be tested by measuring endocrine output associated with the nest coo behavior, and by determining axonal connections of new neurons using combined immunohistochemistry of tract tracing and neuronal markers (Aim II). In sum, the present proposal seeks to determine the role of lesion-induced neurogenesis in the context of the recovery of function in the mature dove brain.

描述（由申请人提供）：我们的长期目标是确定如何帮助脑损伤的成年受害者恢复因中风或其他痛苦而受损的大脑区域的功能。为此，本研究的目标是确定成年大脑受损后功能恢复的机制。本提案以两个概念上互补的意见为基础。我们已经观察到，当雄性环鸽与雌性环鸽一起圈养时，促进了成年雄性环鸽双侧下丘脑损伤后复杂求偶行为（巢咕咕）的恢复。第二，成年大鼠下丘脑电解损伤后，在损伤部位检测到新生神经元，同时在损伤部位对雌性巢叫声刺激表现出正常放电反应的单位在损伤后2-3个月恢复，行为恢复。这些观察结果为广泛接受的功能恢复原则提供了另一种解释。恢复可能不仅由现有的未受损的神经元介导，而且还由损伤诱导的新神经元招募到重组网络中。我们通过阻断神经发生和观察对恢复的影响（目的I）以及确定新神经元是否具有生物学功能，即它们是否整合到巢咕咕行为网络中，来检验损伤诱导的新神经元参与生理活动和巢咕咕行为恢复的假设。这将通过测量与巢咕咕行为相关的内分泌输出，并通过使用联合免疫组化的束示踪和神经元标记物（目的II）确定新神经元的轴突连接来进行测试。总之，本建议旨在确定损伤诱导的神经发生在功能恢复的背景下，在成熟的鸽子脑的作用。