RECONSTITUTION OF TRANSMITTER SECRETION MECHANISMS IN OO

OO中发射机分泌机制的重构

• 批准号: 2332907
• 负责人: PAULO M SERODIO
• 金额: $ 2.44万
• 依托单位: COLUMBIA UNIV NEW YORK MORNINGSIDE
• 依托单位国家: 美国
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-01-04 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/2332907
• 关键词: nerve /myelin protein; neural transmission; neurotransmitter transport; secretion; synapses

The understanding of synaptic transmission and its modulation lies at the very center of our present understanding of brain function. The mechanisms of neurotransmission in synapses have been the subject of many classical studies in physiology. In recent years, several presynaptic proteins have been cloned and characterized by neurobiology research groups. One of the most pressing challenges in the field of synaptic transmission is to establish solid correlations between the roles of known presynaptic proteins and the physiological properties of depolarization-evoked secretion of transmitter. The proposed research aims at developing a model to study transmitter secretion in Xenopus oocytes. For this purpose, I will (1) expand pioneering work on the reconstitution of transmitter secretion in Xenopus oocytes, (2) use of a highly sensitive signal detection protocol consisting of detached, voltage-clamped myocytes and finally, (3) in the context of the reconstitution assay and physiological analysis, to dissect functional roles for cloned presynaptic proteins Synapsin, Synaptophysin, Synaptobrevin and Synaptoporin. The successful reconstitution of secretion mechanisms in oocytes will allow correlation of electrophysiological data with molecular manipulations that can be done in oocytes, not yet possible in other systems.

对突触传递及其调制的理解在于 这是我们目前对大脑功能的理解的中心。这个 突触的神经传递机制一直是许多学者研究的主题 生理学的经典研究。近年来，几个突触前 蛋白质已经被克隆并通过神经生物学研究进行了表征 组。突触领域最紧迫的挑战之一 传播是为了在角色之间建立牢固的关联 已知的突触前蛋白及其生理特性 去极化诱发的递质分泌。 拟议的研究旨在开发一个研究发射机的模型。 非洲爪哇卵母细胞的分泌物。为此，我将(1)展开 非洲爪哇递质分泌重组的开创性工作 卵母细胞，(2)使用高灵敏的信号检测方案 由分离的、电压钳制的心肌细胞组成，最后，(3)在 重建分析和生理分析的上下文，以 剖析克隆的突触前蛋白突触蛋白的功能作用， 突触素、突触素和突触素。成功者 卵母细胞分泌机制的重建将允许相关 电生理数据与分子操作的关系 在卵母细胞中完成，在其他系统中还不可能。