TRANSMISSION OF GRANULOCYTIC EHRLICHIOSIS

粒细胞埃利希体病的传播

• 批准号: 2457850
• 负责人: Sam R Telford
• 金额: $ 22.99万
• 依托单位: HARVARD MEDICAL SCHOOL
• 依托单位国家: 美国
• 财政年份: 1996
• 资助国家: 美国
• 起止时间: 1996-08-01 至 2001-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2457850
• 关键词: Ixodes; Lyme disease; Rickettsiales disease; arthropod borne communicable disease; borreliosis; clinical research; disease /disorder proneness /risk; disease reservoirs; disease vectors; epizootiology; field study; host organism interaction; human subject; immunocytochemistry; intracellular parasitism; laboratory mouse; polymerase chain reaction; serology /serodiagnosis; tissue resource /registry; vertical transmission; vole; western blottings; zoonosis

We propose to determine whether the agent of human granulocytic ehrlichiosis in coastal New England is cotransmitted with those of Lyme disease and human babesiosis. An Ehrlichia with molecular identity to one infecting humans in the upper Midwest was isolated from a Nantucket resident by subinoculation of her blood into laboratory mice. This agent stably infects intact or splenectomised outbred and inbred mice, as well as Peromyscus leucopus, the main enzootic reservoir of Borrelia burgdorferi and Babesia microti in the northeastern U.S. Nymphal deer ticks that had fed as larvae on experimentally infected mice transmitted the agent to uninfected mice, definitively demonstrating vector competence. Thus, we shall 1) determine the mode of perpetuation of the HGE agent in coastal Massachusetts, focusing on the hypothesis that borreliae, babesiae, and ehrlichiae share an enzootic cycle between rodent reservoirs and the deer tick vector; 2) refine our methods for detecting evidence of HGE infection in vertebrate and tick hosts, comparing microscopy-based methods (including immunohistochemistry) with a polymerase chain reaction assay; and 3) describe the public health burden of this emergent zoonosis relative to that of Lyme disease and babesiosis in various coastal New England communities, by retrospective analysis of a large serum bank, as well as by prospective cohort study. Together, these studies are designed to describe how humans become infected by this new zoonotic agent, and provide a basis for intervention.

我们建议确定该制剂是否对人类粒细胞 新英格兰沿海地区的埃立克体病与莱姆病共同传播 疾病和人类巴贝斯虫病。一种具有分子同一性的埃利希体 感染中西部地区北部人类的病毒是从楠塔基特岛的一种病毒中分离出来的 通过将她的血液分接种到实验室小鼠体内来实现驻留。这个代理 也能稳定感染完整或脾脏切除的远交和近交小鼠 作为白鼠白鼠 (Peromyscus leucopus)，疏螺旋体的主要地方性动物宿主 美国东北部的伯氏疏螺旋体和田鼠巴贝斯虫 以幼虫形式喂养实验性感染小鼠的蜱虫传播 未感染小鼠的病原体，明确证明了载体 权限。因此，我们应该 1）确定 马萨诸塞州沿海的 HGE 代理人，重点关注以下假设： 伯氏疏螺旋体、巴贝斯虫和埃利希亚科在 啮齿动物水库和鹿蜱媒介； 2）完善我们的方法 检测脊椎动物和蜱宿主中 HGE 感染的证据， 将基于显微镜的方法（包括免疫组织化学）与 聚合酶链式反应测定； 3) 描述公共卫生 这种新出现的人畜共患疾病的负担相对于莱姆病的负担和 通过回顾性研究，新英格兰各个沿海社区的巴贝斯虫病 对大型血清库的分析以及前瞻性队列研究。 这些研究共同旨在描述人类如何变得 被这种新的人畜共患病病原体感染，并为 干涉。