OPIOID RECEPTORS AND SPINAL NOCICEPTION

阿片受体和脊髓伤害

• 批准号: 2517954
• 负责人: Christopher Nick Honda
• 金额: $ 24.38万
• 依托单位: UNIVERSITY OF MINNESOTA
• 依托单位国家: 美国
• 财政年份: 1995
• 资助国家: 美国
• 起止时间: 1995-09-30 至 1999-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2517954
• 关键词: Macaca; afferent nerve; cell type; confocal scanning microscopy; dorsal root; endogenous opioid; fluorescence microscopy; gene expression; immunocytochemistry; laboratory rat; neuroanatomy; opioid receptor; pain; spinal cord; spinal cord mapping; thalamus

Endogenous opioids modulate neurotransmission through a number of different sensory and motor systems. The present proposal is concerned in general with the modulation of sensory transmission and in particular with opioid modulation of nociceptive sensory systems at the spinal level. The intent of this proposal is to utilize recently developed antibodies to examine in detail the localization of opioid receptors in relation to different functional classes of primary afferent neurons in rats and monkeys. In addition, experiments will determine the spatial relationships between opioid receptors, opioids, and functionally and anatomically identified spinal neurons. In making this proposal, the following specific aims will be addressed: 1. To determine in rats and monkeys what functional classes of primary afferent neurons express opioid receptors. 2. To determine in rats the anatomical and spatial relationships between opioid receptor-containing neuronal elements and retrogradely labeled spinothalamic tract neurons. 3. To determine in monkeys the anatomical and spatial relationships between opioid receptors and functional or morphological subclasses of spinothalamic tract neurons. The presently proposed experiments will determine the anatomical distribution of opioid receptors at a resolution not possible before. Knowledge obtained from these experiments will aid in our understanding of basic opioid mechanisms, and as such will further our understanding of mechanisms related to the modulation of nociceptive and painful information in both the normal and pathologic states.

内源性阿片类物质通过多种途径调节神经传递， 不同的感觉和运动系统目前的建议是， 一般来说，通过调节感觉传递， 在脊髓的伤害性感觉系统的阿片样物质调节 水平该提案的目的是利用最近开发的 抗体，以详细检查阿片受体的定位， 与初级传入神经元不同功能类别的关系 老鼠和猴子此外，实验将确定空间 阿片受体，阿片类药物，功能和 解剖学上鉴定的脊髓神经元在提出这项建议时， 将实现以下具体目标： 1.为了确定在大鼠和猴子中， 传入神经元表达阿片受体。 2.在大鼠中确定 含有阿片受体的神经元元件和逆行标记 脊髓丘脑束神经元 3.为了确定猴子的解剖和空间关系， 阿片受体和功能或形态学亚类之间的关系 脊髓丘脑束神经元 目前提出的实验将确定解剖 阿片受体的分布在以前不可能的分辨率。 从这些实验中获得的知识将有助于我们理解 阿片类药物的基本机制，因此， 与伤害感受和疼痛的调节有关的机制 正常和病理状态下的信息。