OPIOID RECEPTORS AND SPINAL NOCICEPTION

阿片受体和脊髓伤害

• 批准号: 6626824
• 负责人: Christopher Nick Honda
• 金额: $ 21.65万
• 依托单位: UNIVERSITY OF MINNESOTA
• 依托单位国家: 美国
• 财政年份: 1995
• 资助国家: 美国
• 起止时间: 1995-09-30 至 2004-07-09
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6626824
• 关键词: afferent nerve; binding sites; capsaicin; cell component structure /function; cell type; dorsal root; electrical measurement; electrophysiology; fluorescence microscopy; heat stimulus; immunocytochemistry; laboratory rat; lectin; neuroanatomy; neurophysiology; nociceptors; opioid receptor; pain; peripheral nervous system; protein protein interaction; receptor expression; spinal cord; tissue /cell preparation

DESCRIPTION: (Adapted from the Investigator's Abstract) Opioid compounds modulate neurotransmission through a number of different sensory and motor systems. The long-term goals of the present proposal concern the modulation of sensory transmission, especially opioid modulation of nociceptive sensory systems at the spinal level. The immediate intent of this proposal is to concentrate on peripheral mechanisms by examining in detail the localization of delta, mu and kappa opioid receptors to different functional classes of primary afferent neurons. In addition, we will examine relationships between functional properties of afferent neurons and the expression of opioid receptors, vanilloid receptors, and binding sites for the lectin IB4. In making this proposal, immunohistochemical and electrophysiological approaches will be combined to address the following specific aims: Determine what functional classes of primary afferent neurons express opioid receptors. Determine what classes of thermal nociceptors express opioid and vanilloid receptors. Determine in functionally identified sensory neurons, the relationship between the expression of lectin binding sites and opioid and vanilloid receptors. Knowledge obtained from these experiments in the peripheral nervous system will contribute to our understanding of basic opioid mechanisms, and as such will further our understanding of central mechanisms related to the modulation of nociceptive and painful information in both the normal and pathologic states.

描述：（改编自研究者摘要） 阿片类化合物通过多种不同的途径调节神经传递 感觉和运动系统。本提案的长期目标涉及 感觉传递的调节，特别是阿片样物质的调节， 脊髓水平的伤害性感觉系统。这件事的直接意图是 建议通过详细审查 δ、μ和κ阿片样物质受体在不同功能的 初级传入神经元的类别。此外，我们还将研究 传入神经元的功能特性与阿片样物质的表达之间 受体，香草素受体和凝集素IB4的结合位点。作出 这项建议，免疫组织化学和电生理方法将是 合并以实现以下具体目标： 确定哪些功能类别的初级传入神经元表达阿片样物质 受体。 确定哪类热伤害感受器表达阿片类和香草酸 受体。 确定在功能上识别的感觉神经元， 凝集素结合位点和阿片受体及香草素受体的表达。 从这些周围神经系统实验中获得的知识将 有助于我们理解阿片类药物的基本机制，因此， 进一步了解与调节相关的中枢机制 在正常和病理状态下的伤害性和疼痛信息。