OPIOID RECEPTORS AND SPINAL NOCICEPTION

描述：(改编自《调查者摘要》) 阿片类化合物通过多种不同的途径调节神经传递 感觉和运动系统。本提案的长期目标涉及 感觉传递的调节，尤指阿片类物质的调节 脊椎水平上的伤害性感觉系统。这样做的直接目的是 提案的重点是通过详细研究 Delta、Mu和Kappa阿片受体在不同功能中的定位 初级传入神经元的分类。此外，我们还将检查关系 传入神经元功能特性与阿片类药物表达的关系 凝集素IB4的受体、香草素受体和结合部位。在制作中 这项提议、免疫组织化学和电生理方法将是 结合起来以实现以下具体目标： 确定初级传入神经元的哪些功能类别表达阿片 感受器。 确定哪些类别的热伤害性感受器表达阿片和香草素 感受器。 确定在功能鉴定的感觉神经元之间的关系 凝集素结合部位及阿片和香草素受体的表达。 从这些外周神经系统实验中获得的知识将 有助于我们理解阿片类药物的基本机制，并因此将 进一步加深我们对中枢调节机制的理解 正常和病理状态下的伤害性和痛性信息。

DESCRIPTION: (Adapted from the Investigator's Abstract) Opioid compounds modulate neurotransmission through a number of different sensory and motor systems. The long-term goals of the present proposal concern the modulation of sensory transmission, especially opioid modulation of nociceptive sensory systems at the spinal level. The immediate intent of this proposal is to concentrate on peripheral mechanisms by examining in detail the localization of delta, mu and kappa opioid receptors to different functional classes of primary afferent neurons. In addition, we will examine relationships between functional properties of afferent neurons and the expression of opioid receptors, vanilloid receptors, and binding sites for the lectin IB4. In making this proposal, immunohistochemical and electrophysiological approaches will be combined to address the following specific aims: Determine what functional classes of primary afferent neurons express opioid receptors. Determine what classes of thermal nociceptors express opioid and vanilloid receptors. Determine in functionally identified sensory neurons, the relationship between the expression of lectin binding sites and opioid and vanilloid receptors. Knowledge obtained from these experiments in the peripheral nervous system will contribute to our understanding of basic opioid mechanisms, and as such will further our understanding of central mechanisms related to the modulation of nociceptive and painful information in both the normal and pathologic states.