STRUCTURE RELATIONSHIPS OF BRAINSTEM TASTE CELLS

描述(改编自《调查员摘要》)：发展 神经生物学家使用了许多模型来检验这种影响。 感觉输入对中枢神经系统高级神经元成熟的影响。 这种紧张的努力并不令人惊讶，因为更好地理解 这些相互作用对于我们最终理解 大脑的正常发育，以及大脑对 受伤。据推测，发育过程中的形态 大鼠孤束嘴核内的味觉神经元 RAT被修改以响应主节点的性质的变化 传入输入。进一步提出了具体的形态特征。 对特定味觉最敏感的RNST神经元亚群可以 通过减少或增加动物对该物质的暴露而改变 在出生后的早期生活中有味觉。要解决这些问题和相关问题 假设，提出了两个主要的具体目标。第一个目标 将表征结构-功能的正常发展 大鼠RNST中味觉神经元的相互关系。这 信息将为第二个目标提供必要的基础， 这是为了确定更改 味觉环境对结构功能发展的影响 在这个核心中的关系。拟议的研究将通过使用 细胞内记录和标记技术以确定 不同生理学特征的RNST神经元的形态 出生后第10、20-22、28-30天的大鼠。每个神经元都将接受测试 因为它对一系列标准的味觉刺激的反应，然后标记 通过细胞内离子导入注射神经生物素。已标记的 神经元将受到三维质化和 定量分析，将形态特征 与生理反应特性相关的以及由此产生的 结构-功能关系将与前面的关系进行比较 在成年动物身上建立的。然后计划进行研究，以改变 在两个不同的系列实验中，正常的味觉输入到NST。 在第一个系列中，将使用钠剥夺模型来减少 NST内与氯化钠相关的神经元的输入。在另一系列单独的 实验表明，有选择地增加 用0.01M灌胃动物对一种味精的暴露 从出生起每天检查3次盐酸奎宁。在……里面 每个案例都将对RNST的发展进行比较 结构-功能关系和中确定的关系 正常的动物。

DESCRIPTION (Adapted from the Investigator's Abstract): Developmental neurobiologists have employed numerous models to examine the influence of sensory inputs on the maturation of higher-order neurons in the CNS. This intense effort is not surprising, since a better comprehension of these interactions is essential to our ultimate understanding of the normal development of the brain, as well as the brain's response to injury. It is postulated that the morphology of the developing gustatory neurons in the rostral nucleus of the solitary tract (rNST) of the rat is modified in response to changes in the nature of the primary afferent input. It is further proposed that the morphology of specific subsets of rNST neurons that are most sensitive to specific tastants can be altered by decreasing or increasing the animal's exposure to that tastant during early postnatal life. To address these and related hypotheses, two principal Specific Aims are proposed. The first aim will be to characterize the normal development of structure-function relationships of gustatory neurons in the rNST of the rat. This information will provide the necessary foundation for the second aim, which is to determine the effect of altering one component of the gustatory environment on the development of structure-function relationships in this nucleus. Proposed research will begin by using intracellular recording and labeling techniques to determine the morphology of individual physiologically- characterized rNST neurons in rats on postnatal days 10, 20-22 and 28-30. Each neuron will be tested for its response to a standard series of taste stimuli and then labeled by intracellular iontophoretic injection of Neurobiotin. The labeled neurons will be subjected to three-dimensional qualitative and quantitative analysis, the morphological characteristics will be correlated with physiological response properties and the resultant structure-function relationships will be compared to those previously established in adult animals. Studies are then planned to alter the normal gustatory input to the NST in two separate series of experiments. In the first series, a Na-deprivation model will be employed to reduce the NaCl- related input to neurons in the NST. In a separate series of experiments, the developmental impact of selectively increasing the animal's exposure to one tastant by lavaging the oral cavity with 0.01M quinine hydrochloride 3 times each day from birth will be examined. In each case comparisons will be made between the development of rNST structure-function relationships and the relationships identified in normal animals.