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STRUCTURE FUNCTION RELATIONSHIPS IN BRAINSTEM TASTE CELL

脑干味觉细胞的结构功能关系

基本信息

批准号：
6379292
负责人：
WILLIAM E RENEHAN
金额：
$ 32.52万
依托单位：
HENRY FORD HEALTH SYSTEM
依托单位国家：
美国
项目类别：
财政年份：
1992
资助国家：
美国
起止时间：
1992-04-01 至 2002-03-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/6379292
关键词：
GABA receptor afferent nerve developmental neurobiology electron microscopy electrophysiology gamma aminobutyrate immunocytochemistry laboratory rat light microscopy neural transmission receptor expression solitary tract nucleus synapses taste voltage /patch clamp

项目摘要

DESCRIPTION: (Adapted from the Investigator's Abstract) It is well established that gamma-aminoburyric acid (GABA) is an important inhibitory neurotransmitter in virtually every region of the CNS, including the rostral nucleus of the solitary tract (rNST). Interestingly, it appears that the effects of GABA are not limited to the modulation of the activity of mature neurons. Recent data indicate that GABA is also important in the regulation of neural development, first exerting a number of trophic influences on developing brain cells, then serving as an excitatory neurotransmitter and finally an inhibitory neurotransmitter. Unfortunately, we know very little about the factors that stimulate and regulate the developmental changes in GABAergic neurons. There is, however, limited but intriguing evidence that one important factor is the arrival of APPROPRIATE EXCITATORY INPUT. We propose that the protracted postnatal development of the gustatory system offers an excellent opportunity to study this phenomenon. Our studies are based on two hypotheses: 1) GABA functions as an excitatory as well as an inhibitory neurotransmitter during the early postnatal maturation of gustatory neurons in the rNST, with the development of GABA's predominantly inhibitory effect on taste-sensitive rNST neurons coinciding with an increase in the number of type A GABA receptors and a redistribution of type B GABA receptors; 2) The initial disposition and subsequent rearrangement of GABAergic synapses on gustatory neurons is dependent on appropriate primary afferent inputs. We will test these hypotheses with the following experiments: 1)Use light- and electron-microscopic immunohistochemistry to characterize the expression and disposition of type A and type B GABA receptors during postnatal development of the rNST; 2)Determine the effect of GABA and GABA receptor antagonists on the response properties of rNST neurons from early postnatal rats (using in vitro and in vivo recording techniques); 3)Determine the disposition of GABAergic synapses onto identified gustatory neurons during normal development of the rNST; 4)Examine the distribution of GABAergic synapses onto gustatory neurons following transection of the chorda tympani and/or glossopharyngeal nerves. We believe that the studies proposed in this application will yield significant and novel data regarding the development of inhibitory circuits in the central nervous system.

描述：（改编自研究者的摘要）这是公认的 γ-氨基丁酸（GABA）是一种重要的抑制性神经递质几乎遍布中枢神经系统的每个区域，包括嘴核孤立束（rNST）。有趣的是，GABA 的作用似乎是不限于成熟神经元活动的调节。近期数据表明 GABA 在神经发育的调节中也很重要，首先对发育中的脑细胞产生许多营养影响，然后作为兴奋性神经递质，最后作为抑制性神经递质神经递质。不幸的是，我们对影响其影响的因素知之甚少。刺激和调节 GABA 能神经元的发育变化。那里然而，有限但有趣的证据表明，一个重要因素是适当的兴奋输入的到来。我们建议长期出生后味觉系统的发育提供了绝佳的机会来研究这一现象。我们的研究基于两个假设：1) GABA 在神经递质的过程中，既可以作为兴奋性神经递质，也可以作为抑制性神经递质。 rNST 味觉神经元出生后早期成熟， GABA 对味觉敏感 rNST 的主要抑制作用的发展神经元的数量与 A 型 GABA 受体数量的增加同时发生 B 型 GABA 受体的重新分布； 2) 初始处置和随后味觉神经元上 GABA 能突触的重排是依赖于适当的初级传入输入。我们将测试这些假设进行以下实验：1）使用光学和电子显微镜免疫组织化学来表征 A 型的表达和处置出生后 rNST 发育过程中的 B 型 GABA 受体； 2）确定 GABA 和 GABA 受体拮抗剂对反应特性的影响来自出生后早期大鼠的 rNST 神经元（使用体外和体内记录技术）； 3)确定GABA能突触在已识别的 rNST 正常发育过程中的味觉神经元； 4）检查横断后GABA能突触在味觉神经元上的分布鼓索和/或舌咽神经。我们相信本申请中提出的研究将产生重要且新颖的数据关于中枢神经系统抑制回路的发展。

项目成果

期刊论文数量（11）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Redistribution and increased specificity of GABA(B) receptors during development of the rostral nucleus of the solitary tract.

孤束喙核发育过程中 GABA(B) 受体的重新分布和特异性增加。

DOI：
10.1016/s0736-5748(01)00031-4
发表时间：
2001
期刊：
International journal of developmental neuroscience : the official journal of the International Society for Developmental Neuroscience
影响因子：
0
作者：
Heck,WL;Renehan,WE;Schweitzer,L
通讯作者：
Schweitzer,L

Developmental changes in the dendritic architecture of salt-sensitive neurons in the nucleus of the solitary tract.

孤束核中盐敏感神经元树突结构的发育变化。

DOI：
10.1016/s0165-3806(97)00104-1
发表时间：
1997
期刊：
Brain research. Developmental brain research
影响因子：
0
作者：
Renehan,WE;Massey,J;Jin,Z;Zhang,X;Liu,YZ;Schweitzer,L
通讯作者：
Schweitzer,L

Structure and function of gustatory neurons in the nucleus of the solitary tract. III. Classification of terminals using cluster analysis.

孤束核味觉神经元的结构和功能。

DOI：
10.3109/10520299809140523
发表时间：
1998
期刊：
Biotechnic & histochemistry : official publication of the Biological Stain Commission.
影响因子：
0
作者：
Wetherton,BM;Leonard,NL;Renehan,WE;Schweitzer,L
通讯作者：
Schweitzer,L

Cell types in the rostral nucleus of the solitary tract.

孤束喙核的细胞类型。