PROTEIN/RNA INTERACTIONS IN TRANSLATIONAL REGULATION

描述：梅菲尔德博士建议确定这些成分和分子 叶绿体PSBA基因的翻译激活机制。遗传 将在这些研究中使用生化分析，并将进行 在绿藻中，莱茵衣藻。他的总体目标是。 梅菲尔德的实验室将研究原子核和原子核之间的相互作用 细胞器基因组中的协调基因表达。的总体目标 该项目旨在确定用于以下方面的组件和机制 在光中调节特定的叶绿体mRNAs的翻译 依赖的态度。梅菲尔德博士的实验室分离出了 一组与5‘端非翻译区特异结合的蛋白质 氯化PSBA基因。遗传和生化分析表明 这些蛋白质在PSBA mRNA的翻译激活中起作用。这个 莱茵锦鸡儿翻译调控的分子和遗传分析 将有助于理解核/细胞器相互作用的基本原理 协调基因表达，并从独特角度提供洞察力 基因表达的翻译调控的基本方面。 具体目标分为两个方面：一是基因 PSBA翻译所需组件的表征。核基因 PSBA翻译所需的内容将被隔离和表征。以及 PSBA mRNA翻译激活所需的RNA元件为 特色化的。第二个方面是PSBA的生化特性。 RNA结合和起始复合体的形成。拟议中的实验 包括生化事件的特征，这些生化事件导致 光对PSBA RNA结合活性的激活，以及 翻译激活的分子机制表征 由mRNA/蛋白质相互作用引起

DESCRIPTION: Dr. Mayfield proposes to identify the components and molecular mechanism of translational activation of the chloroplast psbA mRNA. Genetic and biochemical analysis will be used in these studies which will be carried out in the green algae, Chlamydomonas reinhardtii. The general aims of Dr. Mayfield's laboratory are to study the interactions of the nuclear and organelle genomes in coordinate gene expression. The general objectives of this project are to identify the components and mechanisms which are used to regulate the translation of specific chloroplastic mRNAs in a light dependent manner. Dr. Mayfield's laboratory has isolated and characterized a set of proteins that bind specifically to the 5' untranslated region of the chloroplastic psbA mRNA. Genetic and biochemical analysis suggests that these proteins act in translational activation of the psbA mRNA. The molecular and genetic analysis of translational regulation in C. reinhardtii will help understand the basic principles in nuclear/organelle interaction in coordinate gene expression, and provide insight from a unique perspective into fundamental aspects of a translational regulation of gene expression. The specific goals are divided into two areas: the first is the genetic characterization of components required for psbA translation. Nuclear genes required for psbA translation will be isolated and characterized. And the RNA elements required for translational activation of psbA mRNA will be characterized. The second area is the biochemical characterization of psbA RNA binding and initiation complex formation. The proposed experiments include the characterization of the biochemical events that lead to activation of psbA RNA binding activity in response to light, and the characterization of the molecular mechanism of translational activation resulting from mRNA/protein interaction