NUCLEAR GENES REGULATING TRANSLATION OF ORGANELLES MRNAS

真核细胞依赖于细胞核和细胞核之间的相互作用。 细胞器基因组的正常生长和发育。 坐标 这些独立的基因组在形成过程中的表达， 线粒体的能量转换复合体， 叶绿体是必需的。 我们正在研究 核和叶绿体基因组在协调表达 通过遗传和分子手段研究光合器官。 一些光合作用蛋白的表达， 叶绿体和核编码的，是由基因调控的 在衣原体叶绿体的生物发生过程中 莱因哈德氏菌 我们发现了一组核突变体 它们缺乏特定叶绿体的翻译 编码的mRNA。 我们将克隆编码这些的核基因 叶绿体跨膜激活剂的互补作用 通过DNA依赖转化的光合突变体， 最近在五月实验室开发的过程。 学习表达 这些跨国激活剂在叶绿体生物发生过程中 应该为理解核武器的作用提供基础， 调控细胞器基因表达的基因。

Eukaryotic cells rely on the interactions of the nuclear and organeller genomes for normal growth and development. Coordinate expression of these independent genomes during the formation of the energy transducing complexes of the mitochondria and chloroplast is essential. We are studying the interactions of nuclear and chloroplast genomes in the coordinate expression of the photosynthetic apparatus by genetic and molecular means. Expression of a number of photosynthetic proteins, both chloroplast and nuclear encoded, is translationally regulated during the biogenesis of the chloroplast in Chlamydomonas reinhardtii. We have characterized a group of nuclear mutants which are deficient in translation of specific chloroplast encoded mRNAs. We will clone the nuclear genes encoding these chloroplast transnational activators by complementation of the photosynthetic mutants via DNA dependent transformation, a process recently developed in may lab. Studying the expression of these transnational activators during chloroplast biogenesis should provide a basis for understanding the role of nuclear genes in regulating organeller gene expression.