PROTEIN/RNA INTERACTIONS IN TRANSLATIONAL REGULATION

描述：Mayfield博士建议鉴定成分和分子 叶绿体psbA mRNA的翻译激活机制。 遗传 和生化分析将用于这些研究， 在绿色海藻里，莱茵衣藻。 博士的目标。 梅菲尔德的实验室将研究原子核和 细胞器基因组在协调基因表达。 的总目标 该项目旨在确定用于 调节特定叶绿体mRNA的翻译， 依赖的方式。 梅菲尔德博士的实验室已经分离出 一组蛋白质，其特异性地结合至蛋白质的5'非翻译区， 叶绿体psbAmRNA。 基因和生化分析表明， 这些蛋白质在psbAmRNA的翻译激活中起作用。 的 对C. reinhardtii 将有助于理解核/细胞器相互作用的基本原理 在协调基因表达方面，并从独特的角度提供见解， 基因表达的翻译调控的基本方面。 具体目标分为两个方面：第一是遗传 psbA翻译所需的组分的表征。 核基因 psbA翻译所需的蛋白质将被分离和表征。 和 psbA mRNA的翻译激活所需的RNA元件将被 表征了 第二个领域是psbA的生化特性 RNA结合和起始复合物形成。 拟议的实验 包括表征导致 响应于光的psbA RNA结合活性的激活，以及 翻译激活的分子机制的表征 mRNA/蛋白质相互作用

DESCRIPTION: Dr. Mayfield proposes to identify the components and molecular mechanism of translational activation of the chloroplast psbA mRNA. Genetic and biochemical analysis will be used in these studies which will be carried out in the green algae, Chlamydomonas reinhardtii. The general aims of Dr. Mayfield's laboratory are to study the interactions of the nuclear and organelle genomes in coordinate gene expression. The general objectives of this project are to identify the components and mechanisms which are used to regulate the translation of specific chloroplastic mRNAs in a light dependent manner. Dr. Mayfield's laboratory has isolated and characterized a set of proteins that bind specifically to the 5' untranslated region of the chloroplastic psbA mRNA. Genetic and biochemical analysis suggests that these proteins act in translational activation of the psbA mRNA. The molecular and genetic analysis of translational regulation in C. reinhardtii will help understand the basic principles in nuclear/organelle interaction in coordinate gene expression, and provide insight from a unique perspective into fundamental aspects of a translational regulation of gene expression. The specific goals are divided into two areas: the first is the genetic characterization of components required for psbA translation. Nuclear genes required for psbA translation will be isolated and characterized. And the RNA elements required for translational activation of psbA mRNA will be characterized. The second area is the biochemical characterization of psbA RNA binding and initiation complex formation. The proposed experiments include the characterization of the biochemical events that lead to activation of psbA RNA binding activity in response to light, and the characterization of the molecular mechanism of translational activation resulting from mRNA/protein interaction