STRUCTURAL STUDIES OF AIDS PROTEINS BY NMR

通过核磁共振对艾滋病蛋白进行结构研究

• 批准号: 2572978
• 负责人: A M GRONENBORN
• 金额: --
• 依托单位: NATIONAL INSTITUTE OF DIABETES AND DIGESTIVE AND KIDNEY DISEASES
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/2572978
• 关键词: AIDS; DNA binding protein; HIV envelope protein; HIV envelope protein gp41; human immunodeficiency virus; human immunodeficiency virus 1; integrase; nuclear magnetic resonance spectroscopy; nucleocapsid; protein structure; structural biology

Work has been carried out on a number of structural problems related to proteins derived from the HIV-1 virus. These include the DNA binding domain of the integrase, the Nef protein and the p7 nucleocapsid protein. The high resolution three-dimensional structures of the dimeric DNA binding domain of HIV-1 integrase and a soluble Nef protein variant have been determined. In addition, work on the n-terminal DNA binding domain of HIV integrase and the trimer forming subdomain of the HIV-1 envelope glycoprotein GP41 is underway.

针对一些结构性问题开展了工作 来自 HIV-1 病毒的蛋白质。 这些包括DNA 整合酶、Nef 蛋白和 p7 的结合域 核衣壳蛋白。 高分辨率三维 HIV-1整合酶的二聚体DNA结合域的结构和 可溶性 Nef 蛋白变体已被确定。 此外，工作 HIV整合酶和三聚体的N端DNA结合域 HIV-1 包膜糖蛋白 GP41 的子结构域的形成正在进行中。