INVESTIGATIONS OF MACROMOLECULAR STRUCTURES AND DYNAMICS IN SOLUTION BY NMR
INVESTIGATIONS OF MACROMOLECULAR STRUCTURES AND DYNAMICS IN SOLUTION BY NMR
批准号:
3754177
负责人:
A M GRONENBORN
金额:
$0.0万
依托单位国家:
美国
项目类别:
财政年份:
--
资助国家:
美国
项目状态:
未结题
起止时间:
至
中文摘要
本实验室的总体研究目标集中在
尽可能完整地描述结构,
溶液中的肽、蛋白质、核酸及其复合物,
主要通过NMR光谱法。目前特别强调的是,
发展的方法,使调查更大的
和复杂的系统,以及提高精度,这些
可以获得溶液结构,研究旨在关联
结构和功能,以及旨在研究蛋白质的实验
折页.
已经确定和分析了几种蛋白质的结构。 这些
包括氧化的和还原的人硫氧还蛋白,
p53结构域、细胞因子hMIP-1-β和蛔虫胰蛋白酶
抑制剂. 这些研究开发了许多新颖的3D和4D
异频NMR实验,以显着提高光谱
分辨率,从而解决较大蛋白质中的分配模糊性。
白细胞介素-8与N-末端细胞质相互作用的位点
其细胞表面受体的一部分已被确定,
链球菌G的IgG结合域的动力学已经被
研究了 最后,对高密度聚乙烯的折叠途径和动力学进行了研究。
链球菌蛋白G的稳定的小IgG结合结构域已经被
研究了
英文摘要
The objective of the overall research in this laboratory is centered on
achieving as complete a description as possible for the structures of
peptides, proteins, nucleic acids and their complexes in solution,
principally by NMR spectroscopy. At present particular emphasis is being
placed on developing approaches which allow the investigation of larger
and complex systems as well as increase the precision with which these
solution structures can be obtained, studies aimed at correlating
structure and function, and experiments aimed at investigating protein
folding.
Structures for several proteins have been determined and analyzed. These
include oxidized and reduced human thioredoxin, the oligomerization
domain of p53, the cytokine hMIP-1-beta, and the Ascaris trypsin
inhibitor. These studies have exploited many novel 3D and 4D
heteronuclear NMR experiments to dramatically increase spectral
resolution and thereby resolve assignment ambiguities in larger proteins.
The site of interaction of interleukin-8 with the N-terminal cytoplasmic
portion of its cell surface receptor has been identified, and the
dynamics of the IgG binding domain of Streptococcal G have been
investigated. Finally, the folding pathway and kinetics of the highly
stable small IgG binding domain of Streptococcal protein G has been
studied.
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会议论文
STRUCTURAL STUDIES OF AIDS PROTEINS BY NMR
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批准号:2572978
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项目类别:
-
资助金额:$0.0万
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财政年份:--
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负责人:A M GRONENBORN
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依托单位:
INVESTIGATIONS OF MACROMOLECULAR STRUCTURES AND DYNAMICS IN SOLUTION BY NMR
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批准号:5201953
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项目类别:
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资助金额:$0.0万
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财政年份:--
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负责人:A M GRONENBORN
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依托单位:
INVESTIGATIONS OF MACROMOLECULAR STRUCTURES IN SOLUTION BY NMR
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批准号:3875845
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项目类别:
-
资助金额:$0.0万
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财政年份:--
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负责人:A M GRONENBORN
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依托单位:
INVESTIGATIONS OF MACROMOLECULAR STRUCTURES AND DYNAMICS IN SOLUTION BY NMR
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批准号:6161937
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项目类别:
-
资助金额:$0.0万
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财政年份:--
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负责人:A M GRONENBORN
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依托单位:
STRUCTURAL STUDIES OF AIDS PROTEINS BY NMR
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批准号:3754175
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项目类别:
-
资助金额:$0.0万
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财政年份:--
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负责人:A M GRONENBORN
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依托单位:
INVESTIGATIONS OF MACROMOLECULAR STRUCTURES AND DYNAMICS IN SOLUTION BY NMR
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批准号:3839839
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项目类别:
-
资助金额:$0.0万
-
财政年份:--
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负责人:A M GRONENBORN
-
依托单位:
STRUCTURAL STUDIES OF AIDS PROTEINS BY NMR
-
批准号:6161935
-
项目类别:
-
资助金额:$0.0万
-
财政年份:--
-
负责人:A M GRONENBORN
-
依托单位:
INVESTIGATIONS OF MACROMOLECULAR STRUCTURES AND DYNAMICS IN SOLUTION BY NMR
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批准号:3776284
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项目类别:
-
资助金额:$0.0万
-
财政年份:--
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负责人:A M GRONENBORN
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依托单位:
STRUCTURAL STUDIES OF AIDS PROTEINS BY NMR
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批准号:5201951
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项目类别:
-
资助金额:$0.0万
-
财政年份:--
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负责人:A M GRONENBORN
-
依托单位:
INVESTIGATIONS OF MACROMOLECULAR STRUCTURES AND DYNAMICS IN SOLUTION BY NMR
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批准号:2572980
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项目类别:
-
资助金额:$0.0万
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财政年份:--
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负责人:A M GRONENBORN
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依托单位:
海外基金