PROTEIN/PROTEIN INTERACTIONS OF HIV 1 INTEGRASE

描述(改编自调查人员摘要)：本项目建议 研究HIV整合酶与宿主的蛋白质相互作用 蛋白。在之前的研究中，PI使用了酵母两种 分离HIV整合酶缺陷突变株的杂交系统 齐聚作用。她还使用了两种混合系统来识别 一种蛋白质，Ini-1，与HIV-1整合酶紧密结合。INI-1转弯 在序列上与SWI/SNF的组件SNF-5相似 复合体被认为与基因激活有关。圆周率计划 研究异构体蛋白质之间的相互作用 利用酵母双杂交系统进行了进一步的实验。变种人会 会影响这些相互作用及其表型 体外和体内研究。INI-1病毒对靶部位的影响 还将调查逆转录病毒整合过程中的选择，因为 INI-1病毒在艾滋病毒生命周期中的作用。希望结果是 这些研究将有助于设计整合抑制剂和 开发新的基因治疗方法。

DESCRIPTION (Adapted from investigator's abstract): This project proposes to investigate protein-protein interactions of HIV integrase with host protein. In previous studies, the PI used the yeast two hybrid system to isolate mutants of HIV integrase defective in oligomerization. She has also used the two hybrid system to identify a protein, Ini-1, that binds tightly to HIV-1 integrase. Ini-1 turns out to be similar in sequence to Snf-5, a component of the Swi/Snf complex proposed to be involved in gene activation. The PI plans to investigate heteromeric protein-protein interactions by further experiments using the yeast two hybrid system. Mutants will be generated that influence these interactions, and their phenotypes studied in vitro and in vivo. The influences of Ini-1 on target site selection during retroviral integration will also be investigated, as will the role of Ini-1 in the HIV life cycle. It is hoped that results of these studies will be useful in devising integration inhibitors and in developing new gene therapy methods.