NEW TRANSDERMAL VACCINE DELIVERY SYSTEM

本研究的目的是论证 一种新的透皮疫苗和/或药物递送方法， 抗原微囊化技术和一种新的电穿孔技术 法 所述方法将允许微球的无痛递送， 角质层（SC）并进入有活力的表皮和真皮。 微囊化提供疫苗抗原的定时释放，它可以 减少了所需抗原的剂量，并能稳定疫苗， 从而延长其保质期。 目前还没有成熟的方法 用于微囊化抗原的透皮递送。 本研究 提出了一种新的透皮方法的发展， 载抗原的微球通过皮肤。 该方法将具有 能够同时给予带电和不带电的微球。 在 第一阶段，微球经皮给药的新方法 含有葡萄球菌肠毒素A的疫苗将被开发和评估 在体外和体内，通过测量免疫反应， 小鼠肠毒素 在第二阶段，将进一步评估该方法， 其它抗原和/或蛋白质和原型装置的递送将 设计了 建议的商业应用：设想新的透皮 用于疫苗和/或药物输送系统将被设计为一次性的 “补丁”，将连接到便携式电压发生器， 注射疫苗或药物。 新系统不会带来痛苦， 便宜又卫生， 在第三世界国家。 此外，该系统还可广泛用于 疾病预防或治疗，其中定时和控制释放 需要将药物注入体液中。

The objective of the proposed research is to demonstrate the feasibility of a new transdermal vaccine and/or drug delivery method which combines the technology of antigen microencapsulation and a novel electroporation method. The method will allow painless delivery of microspheres through the stratum comeum (SC) and into the viable epidermis and dermis. Microencapsulation provides timed release of vaccine antigen, it can reduce the dose of antigen required and it can stabilize the vaccine, thereby prolonging its shelf life. Currently there is no developed method for transdermal delivery of microencapsulated antigens. This research proposes the development of a new transdermal method for the delivery of antigen loaded microspheres through the skin. The method will have the capability of administering both charged and non-charged microspheres. In Phase I, the new method for transdermal delivery of microspheres containing Staphylococcal enterotoxin A will be developed and evaluated both in vitro and in vivo by measuring the immune response to the enteroxin in mice. In Phase II, the method will be further evaluated for the delivery of other antigens and/or proteins and a prototype device will designed. PROPOSED COMMERCIAL APPLICATION: It is envisaged that the new transdermal system for vaccine and/or drug delivery will be designed as a disposable "patch" which will be connected to a portable voltage generator to administer the vaccine or drug. The new system will be painless, inexpensive and sanitary and would have enormous applications for vaccine delivery in Third World countries. Also, the system can be widely used in disease prevention or cure where timed and controlled release of pharmaceuticals into body fluids is needed.