BIOLOGICAL EFFECTS OF JOINT REPLACEMENT

拟议项目的目的是确定是否 接受全髋关节或膝关节置换手术的个人 含有铬的医疗设备在 DNA-蛋白质交联度(DPC)是一种高度敏感的标志物 暴露在铬中造成的早期生物损伤。具体的 目标是： *评估慢性阻塞性肺疾病患者外周血淋巴细胞中DPC水平 手术后关节置换术患者增加。 *评估循环中铬浓度的变化 与DPC水平的变化相关。 *研究手术和水平增加之间的时差 DPC的一部分。 因为髋关节置换术患者已经被观察到 结缔组织肿瘤的发生风险也增加了 与肾癌和前列腺癌一样，DPC检测具有 有可能成为检测任何长期健康状况的监测工具 含铬金属假体装置的后果。 将在接受治疗的患者中招募50名研究参与者 髋关节或膝关节置换手术。他会事先采集血液吗？ 到外科手术，以建立基线测量。每位参与者 将每隔四个周期进行一次为期12个月的随访。在……里面 除了DPC分析外，循环铬浓度将 被测量。

The purpose of the proposed project is to determine whether individuals undergoing total hip or knee replacement procedures with medical devices containing chromium experience an elevation in the levels of DNA-protein crosslinks (DPC), a highly sensitive marker of early biological damige from exposure to chromium. The specific aims are: * To assess whether the levels of DPC in peripheral lymphocytes of joint replacement patients increase after the surgical procedure. * To assess whether changes in circulating chromium concentration correlates with changes in levels of DPC. * To study the time-lag between surgery and the increase in levels of DPC. Since hip replacement patients have already been observed to be at increased risk for development of connective tissue tumors, as well as cancers of the kidney and prostate, the DPC assay has the potential to become a monitoring tool to detect any long-term health consequences from metal prosthetic devices containing chromium. Fifty study participants will be recruited among patients undergoing hip or knee replacement procedures. Blood will he collected prior to surgery to establish a baseline measurement. Each participant will be followed up at four periodic intervals for 12 months. In addition to the DPC assay, circulating chromium concentration will be measured.