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MOLECULAR MARKERS OF HUMAN LIVER CANCER-NOVEL GENES DIFFERENTIALLY EXPRESSED

MOLECULAR MARKERS OF HUMAN LIVER CANCER-NOVEL GENES DIFFERENTIALLY EXPRESSED
人类肝癌的分子标记——差异表达的新基因
批准号：
2463681
负责人：
J A LAUTENBERGER
金额：
$0.0万
依托单位：
DIVISION OF BASIC SCIENCES - NCI
依托单位国家：
美国
项目类别：
财政年份：
--
资助国家：
美国
项目状态：
未结题
起止时间：
至
来源：
https://reporter.nih.gov/project-details/2463681
关键词：
gene expression genetic markers human genetic material tag human tissue liver neoplasms messenger RNA molecular oncology neoplasm /cancer genetics northern blottings polymerase chain reaction tissue /cell culture

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英文摘要
A RNA differential display analysis was performed using RNAs from the liver tumor cell lines SK-HEP-1, HEP-G2 and HEP-3B, as well as normal liver RNA. Promising bands were eluted from the gel and molecularly cloned. About 20 clones were confirmed to correspond to genes that are differentially expressed among the different RNA sources by RNA gel (Northern) blotting. Probing multiple tissue Northern (MTN) blots revealed that several of these genes have limited expression in normal tissues. They may be useful in the study of the biology of liver cancer or may be potential markers for this disease.
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