IDENTIFICATION OF HIV-1 MODULATED GENES

HIV-1 调节基因的鉴定

• 批准号: 2463648
• 负责人: J A LAUTENBERGER
• 金额: --
• 依托单位: DIVISION OF BASIC SCIENCES - NCI
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/2463648
• 关键词: HIV infections; gene expression; genetic library; host organism interaction; human immunodeficiency virus 1; human tissue; macrophage; northern blottings; tissue /cell culture; virulence; virus RNA; virus genetics

HIV-1-induced disease involves the complex interactions of viral and host factors. To better understand the host response to viral infection, the differential display technique was used to identify genes that are differentially expressed between HIV-1-infected and -uninfected macrophages. For several genes, differential expression was confirmed by RNA gel blot (Northern) analysis. The study revealed both genes that are expressed at higher levels in infected cells, as well as ones that are expressed at higher levels in infected cells. Several of these genes had very restricted or undetectable expression in uninfected tissues.

hiv -1诱导的疾病涉及病毒和病毒之间复杂的相互作用