INTRODUCTION OF THE HIV TAT GENE INTO LYMPHOID CELLS

为了研究人类免疫缺陷病毒(HIV)的作用 基因对细胞基因的表达，我们已经构建了淋巴 表达TAT基因的品系。这是通过放置Tat基因来完成的 和邻近的HIV长末端重复序列(LTR)插入逆转录病毒载体 PGV1。将重组表达载体导入PSI2细胞，获得重组腺病毒。 用于感染psiAM细胞的生态型病毒。殖民地 分离G418抗性psiAM细胞并检测其产生 病毒。 使用那些具有最高病毒产量的菌落的上清液 感染人T细胞淋巴系H9的细胞。G418抗性细胞 从H9衍生的品系经检测发现含有TAT活性 与含有LTR和CAT序列的HeLa细胞融合。此外，他们还 包含预测大小的LTR-TAT消息。

In order to study the effect of the human immunodeficiency virus (HIV) tat gene on the expression of cellular genes, we have constructed lymphoid lines that expressed the tat gene. This was done by placing the tat gene and an adjacent HIV long terminal repeat (LTR) into the retroviral vector pGV1. The recombinant plasmid was transfected into psi2 cells to produce an ecotropic viral stock that was used to infect psiAM cells. Colonies of G418-resistant psiAM cells were isolated and assayed for the production of virus. Supernatants of those colonies with the highest virus production were used to infect cells of the human T-cell lymphoid line, H9. G418-resistant cell lines derived from H9 were found to contain tat activity as assayed by fusion with HeLa cells containing LTR and CAT sequences. Furthermore, they contained an LTR-tat message of the predicted size.