IDENTIFICATION OF MOLECULAR MARKERS FOR AUTOIMMUNE DISEASE

自身免疫性疾病分子标记物的鉴定

• 批准号: 3752793
• 负责人: J A LAUTENBERGER
• 金额: --
• 依托单位: DIVISION OF CANCER EPIDEMIOLOGY AND GENETICS
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3752793
• 关键词: Sjogren's syndrome; T lymphocyte; antisense nucleic acid; autoimmune disorder; gene expression; genetic markers; hematopoietic stem cells; human tissue; immunogenetics; laboratory mouse; leukocyte activation disorder; messenger RNA; phenotype; protooncogene; rheumatoid arthritis; scleroderma; systemic lupus erythematosus; tissue /cell culture

The hallmark of autoimmune disease is the production of antibodies to self antigens and presence of activated lymphoid cells at the site of inflammation. Systemic lupus erythematosus (SLE) is characterized by the activation of B- and T-lymphocytes and the production of multiple autoantibodies. The hyperactive B- and T-cells play a major role in the pathogenicity of this disease. We have examined the expression of ets family of genes in peripheral blood mononuclear cells (PBMC) from lupus (SLE), rheumatoid arthritis, scleroderma and Sjogren's syndrome. Both ERGB and ETS2 mRNA were expressed at higher levels in PBMC from SLE patients than healthy individuals. More significantly, ERGB gene expression was high in SLE patients with active disease. The role of ERGB gene products in T-cell activation is being pursued by expressing sense and antisense ERGB cDNA and variant forms of ERGB proteins in T- cells. Molecular markers associated with autoimmune phenotype are being characterized using hematopoietic stem cells derived from normal and autoimmune prone mice.

自身免疫性疾病的特点是产生抗体 自身抗原和活化淋巴细胞的存在 炎。 系统性红斑狼疮 (SLE) 的特点是 B 和 T 淋巴细胞的激活以及多种 自身抗体。 过度活跃的 B 细胞和 T 细胞在 本病的致病性。 我们检查了 ets 的表达 狼疮外周血单核细胞 (PBMC) 中的基因家族 （系统性红斑狼疮）、类风湿性关节炎、硬皮病和干燥综合征。 两个都 ERGB 和 ETS2 mRNA 在 SLE 的 PBMC 中表达水平较高 患者多于健康人。 更重要的是，ERRGB基因 在患有活动性疾病的 SLE 患者中表达较高。 的作用 ERGB基因产物在T细胞激活中的作用正在通过表达来追求 有义和反义 ERGB cDNA 以及 T-中 ERGB 蛋白的变体形式 细胞。 与自身免疫表型相关的分子标记正在被研究 使用源自正常和 自身免疫易感小鼠。