ANTISENSE OLIGONUCLEOTIDES AS INHIBITORS OF BREAST & TUMOR-INDUCED ANGIOGENESIS

作为乳腺抑制剂的反义寡核苷酸

• 批准号: 3752792
• 负责人: J A LAUTENBERGER
• 金额: --
• 依托单位: DIVISION OF CANCER EPIDEMIOLOGY AND GENETICS
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3752792
• 关键词: angiogenesis; antisense nucleic acid; cell cycle; cell differentiation; chemotaxis; complementary DNA; gene expression; gene induction /repression; neoplasm /cancer blood supply; neoplasm /cancer invasiveness; neoplastic process; oligonucleotides; phorbols; protooncogene; vascular endothelium

Tumor progression requires the formation of new capillary blood vessels. It is very likely that anti-angiogenesis agents will be effective in preventing the recurrence of metastasis of many types of tumors. We are investigating the role of the ETS1 proto-oncogene in angiogenesis. We have confirmed that expression of this gene in human umbilical vein endothelial cells (HUVECs) is induced by treatment with phorbol myristic acid. We wish to confirm the role of this gene in angiogenesis by examining the effect of antisense oligonucleotides based on the ets sequence on the behavior of endothelial cells. Properties to be monitored include cell proliferation, differentiation, chemotaxis and invasiveness. We also wish to identify other genes involved in angiogenesis by using the differential display technique to isolate cDNAs corresponding to mRNAs that are expressed differentially between endothelial cells treated and not treated with angiogenic agents.

肿瘤的进展需要形成新的毛细血管。 抗血管生成药物很有可能在 预防多种类型肿瘤的复发转移。我们是 研究ETS1原癌基因在血管生成中的作用。我们 已证实该基因在人脐静脉中的表达 佛波酯诱导内皮细胞的形成 酸。我们希望通过以下方式确认该基因在血管生成中的作用 基于ETS的反义寡核苷酸效应检测 关于内皮细胞行为的序列。财产须为 监测包括细胞增殖、分化、趋化和 侵犯性。我们还希望确定其他涉及到 利用差异显示技术分离cDNA的血管生成 对应于差异表达的mRNAs 血管生成剂治疗和不治疗血管内皮细胞。