ANTISENSE OLIGONUCLEOTIDES AS INHIBITORS OF BREAST & TUMOR-INDUCED ANGIOGENESIS

肿瘤进展需要新的毛细血管的形成。 很可能抗血管生成剂将有效地用于 预防多种肿瘤转移复发。 我们 研究ETS1原癌基因在血管生成中的作用。 我们 已证实该基因在人脐静脉中的表达 内皮细胞（HUVECs）通过用佛波醇肉豆蔻酸酯处理诱导 酸 我们希望证实该基因在血管生成中的作用， 基于ETS检测反义寡核苷酸的作用 序列对内皮细胞行为的影响。 物业 所监测的包括细胞增殖、分化、趋化性和 侵略性 我们还希望鉴定出其他参与 利用差异显示技术分离cDNA， 对应的mRNA表达差异之间 用血管生成剂处理和未处理的内皮细胞。

Tumor progression requires the formation of new capillary blood vessels. It is very likely that anti-angiogenesis agents will be effective in preventing the recurrence of metastasis of many types of tumors. We are investigating the role of the ETS1 proto-oncogene in angiogenesis. We have confirmed that expression of this gene in human umbilical vein endothelial cells (HUVECs) is induced by treatment with phorbol myristic acid. We wish to confirm the role of this gene in angiogenesis by examining the effect of antisense oligonucleotides based on the ets sequence on the behavior of endothelial cells. Properties to be monitored include cell proliferation, differentiation, chemotaxis and invasiveness. We also wish to identify other genes involved in angiogenesis by using the differential display technique to isolate cDNAs corresponding to mRNAs that are expressed differentially between endothelial cells treated and not treated with angiogenic agents.