APPLICATION OF FLOW CYTOMETRY TO CELL BIOLOGY

流式细胞术在细胞生物学中的应用

• 批准号: 2463759
• 负责人: S O SHARROW
• 金额: --
• 依托单位: DIVISION OF BASIC SCIENCES - NCI
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/2463759
• 关键词: T lymphocyte; antigen presentation; apoptosis; biological signal transduction; biomedical resource; cell adhesion molecules; cell differentiation; cell sorting; flow cytometry; graft versus host disease; human tissue; neoplasm /cancer immunology; tissue /cell culture; transplant rejection

The Experimental Immunology Branch flow cytometry laboratory currently supports multiple research projects for more than 40 investigators. These investigations involve quantitative single cell analysis of, and electronic cell separation based upon, parameters associated with cells freshly prepared from different species and/or tissues, as well as a spectrum of in vitro cultured cells. Basic research support is provided to members of the EIB and to other investigators within DBS. Currently supported projects include, but are not limited to, the following areas of study: a) in vivo and in vitro analyses of intra-cellular signaling via T cell surface molecules; b) analyses of cellular processes and/or defects in animals with genetic modifications; c) studies of the pathogenesis of graft-versus host disease; d) analyses of the coordinate cell surface expression of cell adhesion molecules; e) investigations of T cell repertoire generation; g) analyses of expression of transplantation antigens; h) investigations of mechanisms involved in antigen presentation processes; and i) analyses of the mechanisms involved in marrow graft rejection versus acceptance.

实验免疫学分支流式细胞术实验室目前 支持40多名研究人员的多个研究项目。 这些研究涉及定量单细胞分析， 基于细胞相关参数的电子细胞分离 从不同物种和/或组织新鲜制备，以及 体外培养细胞的光谱。 基础研究支持是 提供给EIB成员和DBS内部的其他研究者。 目前支持的项目包括但不限于 研究领域如下：a）在体内和体外分析 通过T细胞表面分子的细胞内信号传导; B）分析 细胞过程和/或缺陷的动物遗传 修改; c）研究移植物抗宿主的发病机制 d）分析细胞的协调细胞表面表达; e）T细胞库生成的研究; g）分析移植抗原的表达; 抗原呈递机制的研究 过程;和i）分析骨髓移植中涉及的机制 拒绝与接受