APPLICATION OF FLOW CYTOMETRY TO CELL BIOLOGY

实验免疫学分院流式细胞仪实验室 支持55名以上研究人员的多个研究项目。这些 研究涉及参数的单细胞定量分析 与从不同物种/组织中新鲜制备的细胞有关，如 以及一系列体外培养的细胞。基础研究支持是 提供给欧洲投资银行的成员以及内部的其他调查人员 DCBDC。目前支持的项目包括但不限于 以下研究领域：a)细胞内的体内和体外分析 通过T细胞表面分子的信号传递，b)分析细胞缺陷 有遗传或诱导免疫功能障碍的动物；c)研究 移植物抗宿主病的发病机制；d)坐标分析 细胞表面黏附分子的表达；e)T细胞的研究 细胞个体发育和分化；f)T细胞机制的研究 曲目生成；g)移植的表达分析 抗原；h)调查涉及抗原呈递的机制 过程；以及i)分析骨髓移植所涉及的机制 拒绝与接受。

The Experimental Immunology Branch flow cytometry laboratory currently supports multiple research projects for more than 55 investigators. These investigations involve quantitative single cell analysis of parameters associated with cells freshly prepared from different species/tissues, as well as a spectrum of in vitro cultured cells. Basic research support is provided to members of the EIB as well as to other investigators within DCBDC. Currently supported projects include, but are not limited to, the following areas of study: a) in vivo and in vitro analyses of intracellular signalling via T cell surface molecules, b) analyses of cellular defects in animals with genetic or induced immune dysfunction; c) studies of the pathogenesis of graft-versus-host disease; d) analyses of the coordinate cell surface expression of cell adhesion molecules; e) investigations of T cell ontogeny and differentiation; f) studies of mechanisms of T cell repertoire generation; g) analyses of expression of transplantation antigens; h) investigations of mechanisms involved in antigen presentation processes; and i) analyses of the mechanisms involved in marrow graft rejection versus acceptance.