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APPLICATION OF FLOW CYTOMETRY TO CELL BIOLOGY

APPLICATION OF FLOW CYTOMETRY TO CELL BIOLOGY
流式细胞术在细胞生物学中的应用
批准号:
5201005
负责人:
S O SHARROW
金额:
$0.0万
依托单位国家:
美国
项目类别:
财政年份:
--
资助国家:
美国
项目状态:
未结题
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中文摘要
翻译
实验免疫学分院流式细胞仪实验室 支持40多名研究人员的多个研究项目。 这些研究包括定量的单细胞分析,以及 基于与参数相关联的电子单元分离 新鲜从不同物种/组织中制备的细胞,以及 体外培养细胞的光谱。基础研究支持是 提供给欧洲投资银行的成员和其他调查人员 DCBDC。目前支持的项目包括但不限于, 以下研究领域:a)体内和体外分析 通过T细胞表面分子的细胞内信号传递;b)分析 遗传性或诱导性疾病动物的细胞过程和/或缺陷 免疫功能障碍或基因工程基因修改; C)移植物抗宿主病的发病机制研究;d) 细胞黏附的坐标细胞表面表示法分析 分子;e)T细胞个体发育、分化和 细胞凋亡;f)T细胞库生成机制的研究; G)分析移植抗原的表达情况;h) 抗原提呈机制的研究 过程;以及i)分析骨髓移植所涉及的机制 拒绝与接受。
英文摘要
The Experimental Immunology Branch flow cytometry laboratory currently supports multiple research projects for more than 40 investigators. These investigations involve quantitative single cell analysis of, and electronic cell separation based upon, parameters associated with cells freshly prepared from different species/tissues, as well as a spectrum of in vitro cultured cells. Basic research support is provided to members of the EIB and to other investigators within DCBDC. Currently supported projects include, but are not limited to, the following areas of study: a) in vivo and in vitro analyses of intra-cellular signalling via T cell surface molecules; b) analyses of cellular processes and/or defects in animals with genetic or induced immune dysfunction or with genetically engineered gene modifications; c) studies of the pathogenesis of graft-versus host disease; d) analyses of the coordinate cell surface expression of cell adhesion molecules; e) investigations of T cell ontogeny, differentiation and apoptosis; f) studies of mechanisms of T cell repertoire generation; g) analyses of expression of transplantation antigens; h) investigations of mechanisms involved in antigen presentation processes; and i) analyses of the mechanisms involved in marrow graft rejection versus acceptance.
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APPLICATION OF FLOW CYTOMETRY TO CELL BIOLOGY
APPLICATION OF FLOW CYTOMETRY TO CELL BIOLOGY
APPLICATION OF FLOW CYTOMETRY TO CELL BIOLOGY
APPLICATION OF FLOW CYTOMETRY TO CELL BIOLOGY
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