APPLICATION OF FLOW CYTOMETRY TO CELL BIOLOGY

实验免疫学分支流式细胞术实验室目前 支持40多名研究人员的多个研究项目。 这些研究涉及定量单细胞分析， 电子细胞分离的基础上，相关参数 从不同物种/组织新鲜制备的细胞，以及 体外培养细胞的光谱。 基础研究支持是 提供给EIB成员和其他调查人员， DCBDC。 目前支持的项目包括，但不限于， 以下研究领域：a）在体内和体外分析 通过T细胞表面分子的细胞内信号传导; B）分析 细胞过程和/或缺陷的动物遗传或诱导 免疫功能障碍或遗传工程基因修饰; c）研究移植物抗宿主病的发病机理; d） 细胞粘附的细胞表面坐标表达分析 e）研究T细胞个体发育、分化和 细胞凋亡; f）T细胞库产生机制的研究; g）分析移植抗原的表达; 抗原呈递机制的研究 过程;和i）分析骨髓移植中涉及的机制 拒绝与接受

The Experimental Immunology Branch flow cytometry laboratory currently supports multiple research projects for more than 40 investigators. These investigations involve quantitative single cell analysis of, and electronic cell separation based upon, parameters associated with cells freshly prepared from different species/tissues, as well as a spectrum of in vitro cultured cells. Basic research support is provided to members of the EIB and to other investigators within DCBDC. Currently supported projects include, but are not limited to, the following areas of study: a) in vivo and in vitro analyses of intra-cellular signalling via T cell surface molecules; b) analyses of cellular processes and/or defects in animals with genetic or induced immune dysfunction or with genetically engineered gene modifications; c) studies of the pathogenesis of graft-versus host disease; d) analyses of the coordinate cell surface expression of cell adhesion molecules; e) investigations of T cell ontogeny, differentiation and apoptosis; f) studies of mechanisms of T cell repertoire generation; g) analyses of expression of transplantation antigens; h) investigations of mechanisms involved in antigen presentation processes; and i) analyses of the mechanisms involved in marrow graft rejection versus acceptance.